Rabbit Anti-PSMD14 Polyclonal Antibody – Cat# MBS649737

PSMD14 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PSMD14 antibody detected the PSMD14 protein (arrow).

PSMD14 Antibody – Rabbit Polyclonal

Name: PSMD14 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649737
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649737

PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]). More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PSMD14, Polyclonal Antibody

Full Product Name

PSMD14, CT (PSMD14, POH1, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1)

Product Gene Name

anti-PSMD14 antibody

Product Synonym Names

Anti -PSMD14, CT (PSMD14, POH1, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

34,577 Da

Chromosome Location

Chromosome: 2; NC_000002.11 (162164786..162268228). Location: 2q24.2

OMIM

607173

3D Structure

ModBase 3D Structure for O00487

Immunogen

PSMD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-287 amino acids from the C-terminal region of human PSMD14.

Product Categories/Family for anti-PSMD14 antibody

Antibodies; Abs to Proteasomes

Applications Tested/Suitable for anti-PSMD14 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-PSMD14 antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-PSMD14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSMD14 purchase

MBS649737 is an antibody targeting PSMD14. Also known as PSMD14, CT (PSMD14, POH1, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PSMD14. It may not necessarily be applicable to this product.

NCBI GI #

16306916

NCBI GeneID

10213

NCBI Accession #

AAH09524.1

UniProt Primary Accession #

O00487

UniProt Secondary Accession #

O00176; B3KNW2

UniProt Related Accession #

O00487

NCBI Official Full Name

PSMD14 protein

NCBI Official Synonym Full Names

proteasome (prosome, macropain) 26S subunit, non-ATPase, 14

NCBI Official Symbol

PSMD14

NCBI Official Synonym Symbols

PAD1; POH1; RPN11

UniProt Protein Name

26S proteasome non-ATPase regulatory subunit 14

UniProt Synonym Protein Names

26S proteasome regulatory subunit RPN11; 26S proteasome-associated PAD1 homolog 1

UniProt Gene Name

PSMD14

UniProt Synonym Gene Names

POH1

UniProt Entry Name

PSDE_HUMAN

NCBI Protein Information

26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1

NCBI Summary for PSMD14

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]

UniProt Comments for PSMD14

Function: Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading. Ref.1 Ref.8 Ref.12

Subunit structure: Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with TXNL1. Ref.8 Ref.9 Ref.12

Tissue specificity: Widely expressed. Highest levels in heart and skeletal muscle. Ref.1

Sequence similarities: Belongs to the peptidase M67A family. PSMD14 subfamily.Contains 1 MPN (JAB/Mov34) domain.

Research Articles on PSMD14

1. The data demonstrated that proteasomal POH1 is a key de-ubiquitinating enzyme that regulates ubiquitin conjugates generated in response to damage and that several aspects of the DNA double-strand break response are regulated by the proteasome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PSMD14 antibody

Reference Product	PubMed Publications
SHFM1 antibody	>1 publications with PSMD14 and SHFM1
UCHL5 antibody	>1 publications with PSMD14 and UCHL5
PSMD7 antibody	>1 publications with PSMD14 and PSMD7

Pathways associated with anti-PSMD14 antibody

Products by Pathway	Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies	APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Activation Of NF-kappaB In B Cells Pathway antibodies	Activation Of NF-kappaB In B Cells Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing-Cross Presentation Pathway antibodies	Antigen Processing-Cross Presentation Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram

Diseases associated with anti-PSMD14 antibody

Disease Name	Pubmed Publications
Death Antibodies	>1 publications with PSMD14 and Death
Ovarian Diseases Antibodies	>1 publications with PSMD14 and Ovarian Diseases
Chromosome Aberrations Antibodies	>1 publications with PSMD14 and Chromosome Aberrations
Aneuploidy Antibodies	>1 publications with PSMD14 and Aneuploidy
Gonadal Disorders Antibodies	>1 publications with PSMD14 and Gonadal Disorders
Weight Loss Antibodies	>1 publications with PSMD14 and Weight Loss
Neoplasms Antibodies	>1 publications with PSMD14 and Neoplasms
Kidney Diseases Antibodies	>1 publications with PSMD14 and Kidney Diseases
Necrosis Antibodies	>1 publications with PSMD14 and Necrosis

Organs/Tissues associated with anti-PSMD14 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with PSMD14 and Blood
Skin Antibodies	>1 publications with PSMD14 and Skin
Brain Antibodies	>1 publications with PSMD14 and Brain