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RFC2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6497436

MBS6497436 is an antibody targeting RFC2. Also known as RFC2, NT (Replication Factor C Subunit 2, Activator 1 Subunit 2, Replication Factor C 40kD Subunit, RF-C 40kD Subunit, RFC40, Activator 1 40kD Subunit, A1 40kD Subunit) (HRP). Reactive in Human. Host:… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RFC2, Polyclonal Antibody
Full Product Name
RFC2, NT (Replication Factor C Subunit 2, Activator 1 Subunit 2, Replication Factor C 40kD Subunit, RF-C 40kD Subunit, RFC40, Activator 1 40kD Subunit, A1 40kD Subunit) (HRP)
Product Gene Name
anti-RFC2 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human RFC2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
35,244 Da
OMIM
194050
3D Structure
ModBase 3D Structure for P35250
Immunogen
Synthetic peptide selected from the N-terminal region of human RFC2 (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-RFC2 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-RFC2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-RFC2 antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-RFC2 antibody

Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)
Rabbit Anti-RFC2 Polyclonal Antibody – Cat# MBS6497436

Western Blot (WB) of anti-RFC2 antibody

Western blot analysis of anti-RFC2 Antibody (N-term) in K562 cell line lysates (35ug/lane). RFC2 (arrow) was detected using the purified Pab.
Rabbit Anti-RFC2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6497436

Flow Cytometry (FC/FACS) of anti-RFC2 antibody

RFC2 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-RFC2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6497436

Product References and Citations for anti-RFC2 antibody

1. Tomida, J., J. Biol. Chem. 283(14), 9071-9079 (2008). 2. Gupte, R.S., Cell Cycle 4(2), 323-329 (2005).

Other Notes

Small volumes of anti-RFC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RFC2. It may not necessarily be applicable to this product.

NCBI GI #
31563534
NCBI GeneID
5982
NCBI Accession #
NP_852136
UniProt Primary Accession #
P35250
UniProt Secondary Accession #
P32846; Q9BU93; B5BU07; D3DXG3
UniProt Related Accession #
P35250
GenBank Nucleotide #
NM_181471
NCBI Official Full Name
replication factor C subunit 2 isoform 1
NCBI Official Synonym Full Names
replication factor C (activator 1) 2, 40kDa
NCBI Official Symbol
RFC2
NCBI Official Synonym Symbols
RFC40
UniProt Protein Name
Replication factor C subunit 2
UniProt Synonym Protein Names
Activator 1 40 kDa subunit; A1 40 kDa subunit; Activator 1 subunit 2; Replication factor C 40 kDa subunit
Protein Family
Probable replication factor
UniProt Gene Name
RFC2
UniProt Synonym Gene Names
A1 40 kDa subunit; RF-C 40 kDa subunit; RFC40
UniProt Entry Name
RFC2_HUMAN
NCBI Protein Information
replication factor C subunit 2; A1 40 kDa subunit; RF-C 40 kDa subunit; activator 1 subunit 2

UniProt Comments for RFC2

RFC2: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; Golgi apparatus; DNA replication factor C complex; nucleus

Molecular Function: protein binding; ATP binding; DNA clamp loader activity

Biological Process: telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; DNA strand elongation during DNA replication; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; DNA repair; DNA replication; telomere maintenance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RFC2 antibody

 Reference Product  PubMed Publications
 PCNA antibody  >35 publications with RFC2 and PCNA
 RFC3 antibody  >26 publications with RFC2 and RFC3
 RFC4 antibody  >26 publications with RFC2 and RFC4
 RFC5 antibody  >21 publications with RFC2 and RFC5
 RFC1 antibody  >18 publications with RFC2 and RFC1
 RAD17 antibody  >8 publications with RFC2 and RAD17
 MCM7 antibody  >1 publications with RFC2 and MCM7

Pathways associated with anti-RFC2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-RFC2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >8 publications with RFC2 and Cardiovascular Diseases
 Heart Diseases Antibodies  >8 publications with RFC2 and Heart Diseases
 Carcinoma Antibodies  >3 publications with RFC2 and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >1 publications with RFC2 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >1 publications with RFC2 and Adenocarcinoma
 Kidney Diseases Antibodies  >1 publications with RFC2 and Kidney Diseases
 Inflammation Antibodies  >1 publications with RFC2 and Inflammation

Organs/Tissues associated with anti-RFC2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with RFC2 and Brain
 Blood Antibodies  >4 publications with RFC2 and Blood
 Heart Antibodies  >2 publications with RFC2 and Heart
 Muscle Antibodies  >2 publications with RFC2 and Muscle
 Adipose Tissue Antibodies  >1 publications with RFC2 and Adipose Tissue
 Liver Antibodies  >1 publications with RFC2 and Liver
 Intestine Antibodies  >1 publications with RFC2 and Intestine
 Kidney Antibodies  >1 publications with RFC2 and Kidney
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