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NCF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649745

MBS649745 is an antibody targeting NCF1. Also known as NCF1, NT (NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, 47kD autosomal chronic granulomatous disease protein, 47kD neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox… More details

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Product Overview

Online Datasheet in PDF
Product Name
NCF1, Polyclonal Antibody
Full Product Name
NCF1, NT (NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, 47kD autosomal chronic granulomatous disease protein, 47kD neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-co
Product Gene Name
anti-NCF1 antibody
Product Synonym Names
Anti -NCF1, NT (NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, 47kD autosomal chronic granulomatous disease protein, 47kD neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-co
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
44,652 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (74188309..74203720). Location: 7q11.23
OMIM
233700
3D Structure
ModBase 3D Structure for P14598
Immunogen
NCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 amino acids from the N-terminal region of human NCF1.

Product Categories/Family for anti-NCF1 antibody

Antibodies; Abs to Enzymes; Oxidase
Applications Tested/Suitable for anti-NCF1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NCF1 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-NCF1 antibody

Western Blot analysis in Ramos cell line lysates (35ug/lane) using MBS649745. This demonstrates that MBS649745 detected the NCF1 protein (arrow).
Rabbit Anti-NCF1 Polyclonal Antibody – Cat# MBS649745

Immunohistochemistry (IHC) of anti-NCF1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS649745 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use MBS649745 for immunohistochemistry.
Rabbit Anti-NCF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649745

Other Notes

Small volumes of anti-NCF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCF1. It may not necessarily be applicable to this product.

NCBI GI #
528078313
NCBI GeneID
653361
NCBI Accession #
NP_000256.4
UniProt Primary Accession #
P14598
UniProt Secondary Accession #
O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
UniProt Related Accession #
P14598
GenBank Nucleotide #
NM_000265.5
NCBI Official Full Name
neutrophil cytosol factor 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; SH3PXD1A
UniProt Protein Name
Neutrophil cytosol factor 1
UniProt Synonym Protein Names
47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Protein Family
Neutrophil cytosol factor
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A; NCF-1
UniProt Entry Name
NCF1_HUMAN
NCBI Protein Information
neutrophil cytosol factor 1; NCF-1; NCF-47K; p47-phox; nox organizer 2; nox-organizing protein 2; NADPH oxidase organizer 2; 47 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 1; SH3 and PX domain-containing protein 1A; 47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)

NCBI Summary for NCF1

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

UniProt Comments for NCF1

Function: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Ref.21

Subunit structure: Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Ref.16 Ref.17 Ref.18 Ref.19 Ref.20

Subcellular location: Cytoplasm Ref.16.

Post-translational modification: Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity. Ref.15 Ref.21

Involvement in disease: Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1) [MIM:233700]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13 Ref.23

Sequence similarities: Contains 1 PX (phox homology) domain.Contains 2 SH3 domains.

Sequence caution: The sequence BAF84783.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG54596.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Research Articles on NCF1

1. three different cross-over points exist within the NCF1 gene cluster, indicating that autosomal p47(phox)-deficient CGD is genetically heterogeneous but can be dissected in detail by MLPA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NCF1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram

Diseases associated with anti-NCF1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >37 publications with NCF1 and Inflammation
 Arthritis, Rheumatoid Antibodies  >22 publications with NCF1 and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >9 publications with NCF1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >6 publications with NCF1 and Heart Diseases
 Necrosis Antibodies  >5 publications with NCF1 and Necrosis
 Hypertension Antibodies  >5 publications with NCF1 and Hypertension
 Liver Diseases Antibodies  >3 publications with NCF1 and Liver Diseases
 Diabetes Mellitus, Experimental Antibodies  >3 publications with NCF1 and Diabetes Mellitus, Experimental
 Hypertrophy Antibodies  >2 publications with NCF1 and Hypertrophy
 Fibrosis Antibodies  >2 publications with NCF1 and Fibrosis

Organs/Tissues associated with anti-NCF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >43 publications with NCF1 and Blood
 Lymph Node Antibodies  >6 publications with NCF1 and Lymph Node
 Spleen Antibodies  >6 publications with NCF1 and Spleen
 Lung Antibodies  >6 publications with NCF1 and Lung
 Lymph Antibodies  >6 publications with NCF1 and Lymph
 Bone Antibodies  >5 publications with NCF1 and Bone
 Brain Antibodies  >4 publications with NCF1 and Brain
 Bone Marrow Antibodies  >2 publications with NCF1 and Bone Marrow
 Connective Tissue Antibodies  >2 publications with NCF1 and Connective Tissue
 Placenta Antibodies  >1 publications with NCF1 and Placenta
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