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EBF-2 (EBF2) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649769

EBF-2 is a 62kD member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate… More details

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Product Overview

Online Datasheet in PDF
Product Name
EBF-2 (EBF2), Polyclonal Antibody
Full Product Name
EBF-2 (Transcription Factor COE2, Early B-cell Factor 2, EBF2, Metencephalon-mesencephalon-olfactory Transcription Factor 1, MET-mesencephalon-olfactory TF1, MET-mesencephalon-olfactory Transcription Factor 1, Olf-1/EBF-like 3, O/E-3, OE-3, Coe2, Mmot1)
Product Gene Name
anti-EBF2 antibody
Product Synonym Names
Anti -EBF-2 (Transcription Factor COE2, Early B-cell Factor 2, EBF2, Metencephalon-mesencephalon-olfactory Transcription Factor 1, MET-mesencephalon-olfactory TF1, MET-mesencephalon-olfactory Transcription Factor 1, Olf-1/EBF-like 3, O/E-3, OE-3, Coe2, Mmot1)
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Mouse
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse EBF-2. Species sequence homology: Recombinant mouse EBF-1 and rmEBF-3 (1%).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
62,650 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (25699246..25902640, complement). Location: 8p21.2
OMIM
609934
3D Structure
ModBase 3D Structure for Q9HAK2
Immunogen
Recombinant corresponding to aa407-519 from mouse EBF-2 expressed in E.coli.

Product Categories/Family for anti-EBF2 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-EBF2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-EBF2 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml

Western Blot (WB) of anti-EBF2 antibody

Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody followed by HRP -conjugated Sheep IgG Secondary Antibody. A specific band was detected for EBF-2 at approximately 65kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Sheep Anti-EBF2 Polyclonal Antibody – Cat# MBS649769

Immunohistochemistry (IHC) of anti-EBF2 antibody

EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody at 10ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Sheep Anti-EBF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649769

Other Notes

Small volumes of anti-EBF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EBF2 purchase

MBS649769 is an antibody targeting EBF2. Also known as EBF-2 (Transcription Factor COE2, Early B-cell Factor 2, EBF2, Metencephalon-mesencephalon-olfactory Transcription Factor 1, MET-mesencephalon-olfactory TF1, MET-mesencephalon-olfactory Transcription Factor 1, Olf-1/EBF-like 3, O/E-3, OE-3, Coe2, Mmot1). Reactive in Mouse. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for EBF2. It may not necessarily be applicable to this product.

NCBI GI #
187953441
NCBI GeneID
64641
NCBI Accession #
AAI37107.1
UniProt Primary Accession #
Q9HAK2
UniProt Secondary Accession #
Q6DK36; Q6IS86; Q6ISA4; A0PJM4; A6NMF7
UniProt Related Accession #
Q9HAK2
NCBI Official Full Name
EBF2 protein
NCBI Official Synonym Full Names
early B-cell factor 2
NCBI Official Symbol
EBF2
NCBI Official Synonym Symbols
COE2; OE-3; EBF-2; O/E-3
UniProt Protein Name
Transcription factor COE2
UniProt Synonym Protein Names
Early B-cell factor 2
Protein Family
EIN3-binding F-box protein
UniProt Gene Name
EBF2
UniProt Synonym Gene Names
COE2; EBF-2
UniProt Entry Name
COE2_HUMAN
NCBI Protein Information
transcription factor COE2; OLF-1/EBF-LIKE 3; Collier, Olf and EBF 2; metencephalon-mesencephalnon-olfactory transcription factor 1

NCBI Summary for EBF2

The protein encoded by this gene belongs to the COE (Collier/Olf/EBF) family of non-basic, helix-loop-helix transcription factors that have a well conserved DNA binding domain. The COE family proteins play an important role in variety of developmental processes. Studies in mouse suggest that this gene may be involved in the differentiation of osteoblasts. [provided by RefSeq, Oct 2011]
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UniProt Comments for EBF2

Function: Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'

By similarity.

Subunit structure: Forms either a homodimer or a heterodimer with a related family member

By similarity.

Subcellular location: Nucleus

Potential.

Sequence similarities: Belongs to the COE family.Contains 1 IPT/TIG domain.

Sequence caution: The sequence BAB13843.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on EBF2

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EBF2 antibody

 Reference Product  PubMed Publications
 TNFRSF11B antibody  >2 publications with EBF2 and TNFRSF11B
 WNT1 antibody  >1 publications with EBF2 and WNT1

Diseases associated with anti-EBF2 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >2 publications with EBF2 and Necrosis
 Disease Models, Animal Antibodies  >2 publications with EBF2 and Disease Models, Animal
 Congenital Abnormalities Antibodies  >2 publications with EBF2 and Congenital Abnormalities
 Developmental Disabilities Antibodies  >1 publications with EBF2 and Developmental Disabilities

Organs/Tissues associated with anti-EBF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >19 publications with EBF2 and Brain
 Embryonic Tissue Antibodies  >8 publications with EBF2 and Embryonic Tissue
 Eye Antibodies  >4 publications with EBF2 and Eye
 Blood Antibodies  >3 publications with EBF2 and Blood
 Muscle Antibodies  >2 publications with EBF2 and Muscle
 Testis Antibodies  >1 publications with EBF2 and Testis
 Vascular Antibodies  >1 publications with EBF2 and Vascular
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