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CPSF3L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649795

The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM… More details

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Product Overview

Online Datasheet in PDF
Product Name
CPSF3L, Polyclonal Antibody
Full Product Name
CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD)
Product Gene Name
anti-CPSF3L antibody
Product Synonym Names
Anti -CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
67,663 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (1246965..1260067, complement). Location: 1p36.33
OMIM
611354
3D Structure
ModBase 3D Structure for Q5TA45
Immunogen
CPSF3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human CPSF3L.

Product Categories/Family for anti-CPSF3L antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-CPSF3L antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CPSF3L antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-CPSF3L antibody

CPSF3L Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF3L antibody detected the CPSF3L protein (arrow).
Rabbit Anti-CPSF3L Polyclonal Antibody – Cat# MBS649795

Immunohistochemistry (IHC) of anti-CPSF3L antibody

CPSF3L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPSF3L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CPSF3L Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS649795

Immunofluorescence (IF) of anti-CPSF3L antibody

Confocal immunofluorescent analysis of CPSF3L Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-CPSF3L Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS649795

Other Notes

Small volumes of anti-CPSF3L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPSF3L purchase

MBS649795 is an antibody targeting CPSF3L. Also known as CPSF3L, NT (CPSF3L, INTS11, RC68, Integrator complex subunit 11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68kD). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CPSF3L. It may not necessarily be applicable to this product.

NCBI GI #
39645207
NCBI GeneID
54973
NCBI Accession #
AAH13904.2
UniProt Primary Accession #
Q5TA45
UniProt Secondary Accession #
Q5TA46; Q5TA48; Q5TA52; Q5TA53; Q5TA54; Q7L3D7; Q96HY1; A8K5S2; B3KPR3; B4DM87; G3V1S5
UniProt Related Accession #
Q5TA45
NCBI Official Full Name
CPSF3L protein, partial
NCBI Official Synonym Full Names
cleavage and polyadenylation specific factor 3-like
NCBI Official Symbol
CPSF3L
NCBI Official Synonym Symbols
RC68; INT11; RC-68; INTS11; CPSF73L
UniProt Protein Name
Integrator complex subunit 11
UniProt Synonym Protein Names
Cleavage and polyadenylation-specific factor 3-like protein; CPSF3-like protein; Protein related to CPSF subunits of 68 kDa
UniProt Gene Name
CPSF3L
UniProt Synonym Gene Names
INTS11; RC68; Int11; CPSF3-like protein; RC-68
UniProt Entry Name
INT11_HUMAN
NCBI Protein Information
integrator complex subunit 11; CPSF3-like protein; related to CPSF subunits 68 kDa; protein related to CPSF subunits of 68 kDa; cleavage and polyadenylation-specific factor 3-like protein

UniProt Comments for CPSF3L

Function: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Ref.7

Subunit structure: Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Ref.7

Subcellular location: Nucleus. Cytoplasm Ref.8.

Domain: The HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex

By similarity.

Sequence similarities: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.

Sequence caution: The sequence BAA91246.1 differs from that shown. Reason: Frameshift at position 587. The sequence BAB14541.1 differs from that shown. Reason: Frameshift at position 248. The sequence CAI23174.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23176.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23177.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI23179.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23180.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23181.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on CPSF3L

1. The specific heterodimeric interaction between IntS9 and IntS11 is mediated by a discrete domain present at the extreme C terminus of IntS9 and within the C terminus of IntS11, adjacent to the predicted active site of this endonuclease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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