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Ribonuclease P Protein Subunit P20 (POP7) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649830

The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs… More details

$1405 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
Ribonuclease P Protein Subunit p20 (POP7), Monoclonal Antibody
Full Product Name
Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)
Product Gene Name
Product Synonym Names
Anti -Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5BH5
Host
Mouse
Form/Format
Supplied as a lyophilized powder from 10mM ammonium bicarbonate buffer, 2mg BSA. Reconstitute with sterile dH2O.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Ribonuclease P Protein Subunit p20.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Molecular Weight
15,651 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (100303676..100305123). Location: 7q22
OMIM
606113
3D Structure
ModBase 3D Structure for O75817
Immunogen
Recombinant GST-hRPP20 (Accession #AAC24113, expression vector pGEX-2T), expressed in E.coli.
Applications Tested/Suitable for anti-POP7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-POP7 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: Under less stringent washing conditions, a weaker band of ~60kD is also detected.

Other Notes

Small volumes of anti-POP7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POP7 purchase

MBS649830 is an antibody targeting POP7. Also known as Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for POP7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribonuclease P protein subunit p20
NCBI Official Synonym Full Names
processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae)
NCBI Official Symbol
POP7
NCBI Official Synonym Symbols
RPP2; RPP20; 0610037N12Rik
UniProt Protein Name
Ribonuclease P protein subunit p20
UniProt Synonym Protein Names
Ribonucleases P/MRP protein subunit POP7 homolog
UniProt Gene Name
POP7
UniProt Synonym Gene Names
RPP20; RNaseP protein p20; hPOP7
UniProt Entry Name
POP7_HUMAN
NCBI Protein Information
ribonuclease P protein subunit p20; hPOP7; RNaseP protein p20; ribonucleases P/MRP protein subunit POP7 homolog; processing of precursor 7, ribonuclease P subunit; POP7 (processing of precursor, S. cerevisiae) homolog

UniProt Comments for POP7

Function: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.

Catalytic activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

Subunit structure: Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38 and RPP40. RNase MRP consists of a RNA moiety and at least 9 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38, RPP40, POP5 and RPP21. Interacts with RPP25. RPP20/POP7 is probably a dimer. Ref.5

Subcellular location: Nucleus › nucleolus

Potential.

Sequence similarities: Belongs to the histone-like Alba family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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