Rabbit Anti-DCPS Polyclonal Antibody – Cat# MBS649862

DCPS Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DCPS antibody detected the DCPS protein (arrow).

DCPS Antibody – Rabbit Polyclonal

Name: DCPS Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS649862
Price: 895 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS649862

Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA… More details

$895 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DCPS, Polyclonal Antibody

Full Product Name

DCPS, ID (DCPS, DCS1, HINT5, m7GpppX diphosphatase, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, Scavenger mRNA-decapping enzyme DcpS)

Product Gene Name

anti-DCPS antibody

Product Synonym Names

Anti -DCPS, ID (DCPS, DCS1, HINT5, m7GpppX diphosphatase, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, Scavenger mRNA-decapping enzyme DcpS)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Human

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

38,402 Da

Chromosome Location

Chromosome: 29; AC_000186.1 (30022608..30065785). Location: chromosome: 29

3D Structure

ModBase 3D Structure for Q8MJJ7

Immunogen

DCPS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the Central region of human DCPS.

Product Categories/Family for anti-DCPS antibody

Antibodies; Abs to Enzymes; Phosphatase

Applications Tested/Suitable for anti-DCPS antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-DCPS antibody

Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500

Other Notes

Small volumes of anti-DCPS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCPS purchase

MBS649862 is an antibody targeting DCPS. Also known as DCPS, ID (DCPS, DCS1, HINT5, m7GpppX diphosphatase, DCS-1, Hint-related 7meGMP-directed hydrolase, Histidine triad protein member 5, Scavenger mRNA-decapping enzyme DcpS). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DCPS. It may not necessarily be applicable to this product.

NCBI GI #

146186594

NCBI GeneID

282640

NCBI Accession #

AAI40690.1

UniProt Primary Accession #

Q8MJJ7

UniProt Related Accession #

Q8MJJ7

NCBI Official Full Name

DCPS protein

NCBI Official Synonym Full Names

decapping enzyme, scavenger

NCBI Official Symbol

DCPS

NCBI Official Synonym Symbols

HINT-5

UniProt Protein Name

m7GpppX diphosphatase

UniProt Synonym Protein Names

DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS

Protein Family

m7GpppX diphosphatase

UniProt Gene Name

DCPS

UniProt Synonym Gene Names

DCS1; HINT5; HINT-5

UniProt Entry Name

DCPS_BOVIN

NCBI Protein Information

m7GpppX diphosphatase; DCS-1; mRNA decapping enzyme; decapping scavenger enzyme; histidine triad protein member 5; scavenger mRNA-decapping enzyme DcpS; hint-related 7meGMP-directed hydrolase; histidine triad nucleotide-binding protein 5

UniProt Comments for DCPS

Function: Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death

By similarity.

Catalytic activity: A 5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA) + H2O = N(7)-methylguanosine 5'-phosphate + a 5'-diphospho-(mRNA).

Enzyme regulation: The hydrolytic product 7-methylguanosine diphosphate (m7GDP) efficiently inhibits the decapping scavenger activity and acts as a competitive inhibitor in vitro. Inhibited by 2,4-diaminoquinazoline

By similarity.

Subunit structure: Homodimer. Associates with components of the exosome multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts with NDOR1

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Note: Predominantly localized in the nucleus. Nucleocytoplasmic shuttling protein that can transiently enter the cytoplasm in mammalian cells in a XPO1/CRM1-dependent manner

By similarity.

Domain: The C-terminal histidine triad (HIT) motif and the N-terminal domain are required for the decapping activity. The N-terminus is necessary but not sufficient for binding cap structures

By similarity.

Sequence similarities: Belongs to the HIT family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DCPS antibody

Products by Pathway	Pathway Diagram
Deadenylation-dependent MRNA Decay Pathway antibodies	Deadenylation-dependent MRNA Decay Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram
MRNA Decay By 3' To 5' Exoribonuclease Pathway antibodies	MRNA Decay By 3' To 5' Exoribonuclease Pathway Diagram

Organs/Tissues associated with anti-DCPS antibody

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>1 publications with DCPS and Ovary