Interleukin 10 (IL10) Active Protein

Name: IL10 Active Protein; Interleukin 10; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650143
Price: 485 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650143

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor More details

$485 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 10 (IL10), Active Protein

Full Product Name

Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Product Gene Name

IL10 active protein

Sequence

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Form/Format

Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.2, without additives. Reconstitute with 5mM Tris, pH 7.2 to a concentration of 0.1-1mg/ml.

Purity/Purification

Molecular Biology Grade
98% by SDS-PAGE and HPLC

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

20,517 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (206940948..206945839, complement). Location: 1q31-q32

OMIM

124092

3D Structure

ModBase 3D Structure for P22301

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5x10e5 units/mg.

Country of Origin

USA

Endotoxin Level

0.1ng/ug

Product Categories/Family for IL10 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL10 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL10 purchase

MBS650143 is an active protein targeting IL10. Also known as Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF).

Additional Details

NCBI/Uniprot data below describe general gene information for IL10. It may not necessarily be applicable to this product.

NCBI GI #

10835141

NCBI GeneID

3586

NCBI Accession #

NP_000563.1

UniProt Primary Accession #

P22301

UniProt Related Accession #

P22301; Q6FGS9; Q6FGW4; Q6LBF4; Q71UZ1; Q9BXR7

GenBank Nucleotide #

NM_000572.2

NCBI Official Full Name

interleukin-10

NCBI Official Synonym Full Names

interleukin 10

NCBI Official Symbol

IL10

NCBI Official Synonym Symbols

CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451

UniProt Protein Name

Interleukin-10

UniProt Synonym Protein Names

Cytokine synthesis inhibitory factor

Protein Family

Interleukin

UniProt Gene Name

IL10

UniProt Entry Name

IL10_HUMAN

NCBI Protein Information

interleukin-10; OTTHUMP00000034567; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor

NCBI Summary for IL10

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

UniProt Comments for IL10

Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Sequence similarities: Belongs to the IL-10 family.

Research Articles on IL10

1. concentration of IL-1beta, INF-gamma, TNF-alpha, S100beta, AMA-M2 increase, IL-10 decrease were detected in peripheral blood of 25 patients with stroke

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL10 active protein

Reference Product	PubMed Publications
IL10RB active protein	>28 publications with IL10 and IL10RB
IL10RA active protein	>13 publications with IL10 and IL10RA

Pathways associated with IL10 active protein

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram

Diseases associated with IL10 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>4544 publications with IL10 and Inflammation
Neoplasms Antibodies	>1819 publications with IL10 and Neoplasms
Lung Diseases Antibodies	>1176 publications with IL10 and Lung Diseases
Intestinal Diseases Antibodies	>1122 publications with IL10 and Intestinal Diseases
Sepsis Antibodies	>920 publications with IL10 and Sepsis
Liver Diseases Antibodies	>756 publications with IL10 and Liver Diseases
Colitis Antibodies	>654 publications with IL10 and Colitis
Hypersensitivity, Immediate Antibodies	>640 publications with IL10 and Hypersensitivity, Immediate
Asthma Antibodies	>506 publications with IL10 and Asthma
Arthritis, Rheumatoid Antibodies	>504 publications with IL10 and Arthritis, Rheumatoid

Organs/Tissues associated with IL10 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>12427 publications with IL10 and Blood
Spleen Antibodies	>1497 publications with IL10 and Spleen
Lung Antibodies	>1333 publications with IL10 and Lung
Connective Tissue Antibodies	>148 publications with IL10 and Connective Tissue