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Fibroblast Growth Factor, Basic Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650174

Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. It binds to heparin, which potentiates its biological activity and protects it from proteolysis. The growth factor is… More details

$370 / 0.002 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor, Basic, Active Protein
Full Product Name
Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gl y-Ser-Ile.
Host
Sf9 insect cells
Form/Format
Supplied as a liquid in 20mM Tris pH 7.9, 100mM KCl, 1mM DTT and 20% glycerol.
Purity/Purification
Highly Purified
98.0% as determined by:
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
17.35kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Recombinant Human FGF-b is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 2x10e6Units/mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of FGF-basic-Sf9.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of FGF-b as a Reference Standard.

Other Notes

Small volumes of Fibroblast Growth Factor, Basic active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor, Basic purchase

MBS650174 is an active protein targeting Fibroblast Growth Factor, Basic. Also known as Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin). Host: Sf9 insect cells.
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