Interleukin 2 (IL2) Active Protein

Name: IL2 Active Protein; Interleukin 2; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650191
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650191

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of… More details

$370 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 2 (IL2), Active Protein

Full Product Name

Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF)

Product Gene Name

IL2 active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pr o-Thr-Ser.

Host

E Coli

Form/Format

Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute in sterile 20mM acetic acid to a concentration of 0.1mg/ml.

Purity/Purification

Highly Purified
~98% by RP-HPLC and SDS-PAGE. Chromatographically purified.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 20mM acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

17,628 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (123372625..123377650, complement). Location: 4q26-q27

OMIM

147680

3D Structure

ModBase 3D Structure for P60568

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is
< 0.0645ng/ml, corresponding to a Specific Activity of 15,500,000IU/mg.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Product Categories/Family for IL2 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL2 purchase

MBS650191 is an active protein targeting IL2. Also known as Interleukin 2, Recombinant, Human (Interleukin-2, IL-2, IL2, Aldesleukin, Lymphokine, T-cell Growth Factor, TCGF). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL2. It may not necessarily be applicable to this product.

NCBI GI #

512412

NCBI GeneID

3558

UniProt Primary Accession #

P60568

UniProt Secondary Accession #

P01585

UniProt Related Accession #

P60568; Q0GK43; Q13169; Q16334; Q309Q7; Q6NZ91; Q6NZ93; Q6QWN0; Q71V48; Q7Z7M3

NCBI Official Full Name

interleukin-2

NCBI Official Synonym Full Names

interleukin 2

NCBI Official Symbol

IL2

NCBI Official Synonym Symbols

IL-2; TCGF; lymphokine

UniProt Protein Name

Interleukin-2

UniProt Synonym Protein Names

T-cell growth factor

Protein Family

Interleukin

UniProt Gene Name

IL2

UniProt Entry Name

IL2_HUMAN

NCBI Protein Information

interleukin-2; aldesleukin; OTTHUMP00000164090; T cell growth factor; involved in regulation of T-cell clonal expansion

NCBI Summary for IL2

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq]

UniProt Comments for IL2

Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Subcellular location: Secreted.

Involvement in disease: Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

Pharmaceutical use: Available under the name Proleukin (Chiron). Used in patients with renal cell carcinoma or metastatic melanoma.

Sequence similarities: Belongs to the IL-2 family.

Sequence caution: The sequence AAA59140.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on IL2

1. Data show there was a significant decrease in Th1 intracellular cytokines IFNgamma, IL-2 and TNFalpha in the blood of culture positive patients compared with culture negative patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL2 active protein

Reference Product	PubMed Publications
IL2RA active protein	>68 publications with IL2 and IL2RA
IL2RB active protein	>28 publications with IL2 and IL2RB
IL2RG active protein	>5 publications with IL2 and IL2RG
NGFR active protein	>1 publications with IL2 and NGFR

Pathways associated with IL2 active protein

Products by Pathway	Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with IL2 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>699 publications with IL2 and Neoplasms
Necrosis Antibodies	>244 publications with IL2 and Necrosis
Autoimmune Diseases Antibodies	>203 publications with IL2 and Autoimmune Diseases
Leukemia Antibodies	>198 publications with IL2 and Leukemia
Carcinoma Antibodies	>171 publications with IL2 and Carcinoma
Lymphoma Antibodies	>146 publications with IL2 and Lymphoma
Neoplasms, Experimental Antibodies	>129 publications with IL2 and Neoplasms, Experimental
Inflammation Antibodies	>126 publications with IL2 and Inflammation
Melanoma Antibodies	>102 publications with IL2 and Melanoma
Kidney Diseases Antibodies	>94 publications with IL2 and Kidney Diseases

Organs/Tissues associated with IL2 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1837 publications with IL2 and Blood
Uncharacterized Tissue Antibodies	>1 publications with IL2 and Uncharacterized Tissue