Interleukin 20 (IL20) Active Protein

Name: IL20 Active Protein; Interleukin 20; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650218
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650218

The newly discovered cytokine interleukin-20 (IL-20) is structurally related to IL-10, yet it appears to be an autocrine factor for keratinocytes that regulates their participation in inflammation. Recombinant Human IL-20 is a single, non-glycosylated… More details

$370 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 20 (IL20), Active Protein

Full Product Name

Interleukin 20, Recombinant, Human (IL-20)

Product Gene Name

IL20 active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Ly s-Thr-Leu.

Host

Recombinant, Human from E Coli

Form/Format

Supplied as a lyophilized powder in 100mM sodium chloride, 10mM sodium citrate, pH 3.0.

Purity/Purification

Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

20,072 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (207038699..207042568). Location: 1q32

OMIM

605619

3D Structure

ModBase 3D Structure for Q9NYY1

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

IL-20 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human MO7e cells is < 0.2ng/ml, corresponding to a Specific Activity of 5x10e6 IU/mg.

Dimers and Aggregates

1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of hIL-20.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of IL-20 as a Reference Standard.

Reconstitution

Reconstitute the lyophilized Interleukin-20 in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for IL20 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL20 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL20 purchase

MBS650218 is an active protein targeting IL20. Also known as Interleukin 20, Recombinant, Human (IL-20). Host: Recombinant, Human from E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL20. It may not necessarily be applicable to this product.

NCBI GI #

109658898

NCBI GeneID

50604

NCBI Accession #

AAI17391.1

UniProt Primary Accession #

Q9NYY1

UniProt Secondary Accession #

Q17RB3; Q96QZ6

UniProt Related Accession #

Q9NYY1

NCBI Official Full Name

Interleukin 20

NCBI Official Synonym Full Names

interleukin 20

NCBI Official Symbol

IL20

NCBI Official Synonym Symbols

IL-20; IL10D; ZCYTO10

UniProt Protein Name

Interleukin-20

UniProt Synonym Protein Names

Cytokine Zcyto10

Protein Family

Interleukin

UniProt Gene Name

IL20

UniProt Synonym Gene Names

ZCYTO10; IL-20

UniProt Entry Name

IL20_HUMAN

NCBI Protein Information

interleukin-20; cytokine Zcyto10; four alpha helix cytokine

NCBI Summary for IL20

The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]

UniProt Comments for IL20

Function: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.

Subcellular location: Secreted.

Tissue specificity: Expressed at very low levels in skin, trachea, and other tissues.

Sequence similarities: Belongs to the IL-10 family.

Research Articles on IL20

1. The mRNA levels of p40, IL-20 and IL-21 at baseline may serve as potential predictors of treatment response to ustekinumab treatment in psoriasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL20 active protein

Reference Product	PubMed Publications
IL20RB active protein	>2 publications with IL20 and IL20RB
IL20RA active protein	>2 publications with IL20 and IL20RA
IL22RA1 active protein	>1 publications with IL20 and IL22RA1

Pathways associated with IL20 active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with IL20 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>3 publications with IL20 and Inflammation
Necrosis Antibodies	>2 publications with IL20 and Necrosis
Lung Diseases Antibodies	>2 publications with IL20 and Lung Diseases
Pulmonary Fibrosis Antibodies	>1 publications with IL20 and Pulmonary Fibrosis
Carcinoma, Hepatocellular Antibodies	>1 publications with IL20 and Carcinoma, Hepatocellular
Brain Diseases Antibodies	>1 publications with IL20 and Brain Diseases
Diabetes Mellitus, Type 1 Antibodies	>1 publications with IL20 and Diabetes Mellitus, Type 1