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Midkine (MDK) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650351

Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogensis and has several… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Midkine (MDK), Recombinant Protein
Full Product Name
Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2)
Product Gene Name
Host
E Coli
Form/Format
Supplied as a sterile filtered, lyophilized powder from PBS, pH 7.4. Reconstitute with sterile dH2O, carrier protein to 0.1-1mg/ml
Purity/Purification
Molecular Biology Grade
98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile dH2O, and a carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
13.4kD
Chromosome Location
Chromosome: 11; NC_000011.9 (46402618..46405375). Location: 11p11.2
OMIM
162096
3D Structure
ModBase 3D Structure for P21741
Country of Origin
USA

Other Notes

Small volumes of MDK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDK purchase

MBS650351 is a recombinant protein targeting MDK. Also known as Midkine, Recombinant, Human (MK, Neurite Growth Promoting Factor-2, NEGF-2). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MDK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
midkine
NCBI Official Synonym Full Names
midkine (neurite growth-promoting factor 2)
NCBI Official Symbol
MDK
NCBI Official Synonym Symbols
MK; NEGF2; FLJ27379
UniProt Protein Name
Midkine
UniProt Synonym Protein Names
Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
Protein Family
UniProt Gene Name
MDK
UniProt Synonym Gene Names
MK1; NEGF2
UniProt Entry Name
MK_HUMAN
NCBI Protein Information
midkine; ARAP; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197043; OTTHUMP00000197044; OTTHUMP00000197045; OTTHUMP00000233051; OTTHUMP00000233088; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor 2

NCBI Summary for MDK

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).[supplied by OMIM]
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UniProt Comments for MDK

Function: Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development

By similarity. Ref.2

Subunit structure: Homodimer. Ref.9

Subcellular location: Secreted.

Induction: By heparin and retinoic acid. Ref.2 Ref.7

Sequence similarities: Belongs to the pleiotrophin family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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