Interleukin 7 (IL7) Active Protein

Name: IL7 Active Protein; Interleukin 7; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650361
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650361

Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and… More details

$425 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 7 (IL7), Active Protein

Full Product Name

Interleukin 7, Recombinant, Mouse (IL-7)

Product Gene Name

IL7 active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cy s-His-Ile.

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives.

Purity/Purification

Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

-20 degree C
Stability: Lyophilized Recombinant Murine IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Murine IL-7 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight

14.90kD

Chromosome Location

Chromosome: 8; NC_000008.10 (79645007..79717758, complement). Location: 8q12-q13

OMIM

146660

3D Structure

ModBase 3D Structure for P13232

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Murine IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.

Dimers and Aggregates

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of Murine IL-7.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Murine IL-7 as a Reference Standard.

Solubility

It is recommended to reconstitute the lyophilized Murine IL-7 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for IL7 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL7 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL7 purchase

MBS650361 is an active protein targeting IL7. Also known as Interleukin 7, Recombinant, Mouse (IL-7). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL7. It may not necessarily be applicable to this product.

NCBI GI #

315467869

NCBI GeneID

3574

NCBI Accession #

NP_001186816.1

UniProt Primary Accession #

P13232

UniProt Secondary Accession #

Q5FBY5; Q5FBY9; A0N0L3

UniProt Related Accession #

P13232

GenBank Nucleotide #

NM_001199887.1

NCBI Official Full Name

interleukin-7 isoform 3

NCBI Official Synonym Full Names

interleukin 7

NCBI Official Symbol

IL7

NCBI Official Synonym Symbols

IL-7

UniProt Protein Name

Interleukin-7

Protein Family

Interleukin

UniProt Gene Name

IL7

UniProt Synonym Gene Names

IL-7

UniProt Entry Name

IL7_HUMAN

NCBI Protein Information

interleukin-7

NCBI Summary for IL7

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

UniProt Comments for IL7

Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-7/IL-9 family.

Research Articles on IL7

1. genetic, immunologic, and metabolic factors contribute to a dysregulation of the IL-7/IL-7 receptor pathway in type 1 diabetes and identify a novel hyperglycemia-mediated interference of immune regulatory networks.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL7 active protein

Reference Product	PubMed Publications
IL7R active protein	>12 publications with IL7 and IL7R
IL3 active protein	>9 publications with IL7 and IL3
IL2RG active protein	>9 publications with IL7 and IL2RG
CD19 active protein	>8 publications with IL7 and CD19
JAK3 active protein	>8 publications with IL7 and JAK3
IL2RA active protein	>7 publications with IL7 and IL2RA
IL9 active protein	>7 publications with IL7 and IL9
CSF2 active protein	>3 publications with IL7 and CSF2
JAK1 active protein	>2 publications with IL7 and JAK1

Pathways associated with IL7 active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram

Diseases associated with IL7 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>60 publications with IL7 and Neoplasms
Inflammation Antibodies	>20 publications with IL7 and Inflammation
Necrosis Antibodies	>18 publications with IL7 and Necrosis
Lymphopenia Antibodies	>17 publications with IL7 and Lymphopenia
Nervous System Diseases Antibodies	>16 publications with IL7 and Nervous System Diseases
Multiple Sclerosis Antibodies	>15 publications with IL7 and Multiple Sclerosis
Neoplasms, Experimental Antibodies	>14 publications with IL7 and Neoplasms, Experimental
Carcinoma Antibodies	>9 publications with IL7 and Carcinoma
Hypersensitivity Antibodies	>6 publications with IL7 and Hypersensitivity
Cell Transformation, Neoplastic Antibodies	>6 publications with IL7 and Cell Transformation, Neoplastic

Organs/Tissues associated with IL7 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>183 publications with IL7 and Blood
Bone Antibodies	>46 publications with IL7 and Bone
Thymus Antibodies	>37 publications with IL7 and Thymus
Lymph Node Antibodies	>19 publications with IL7 and Lymph Node
Skin Antibodies	>11 publications with IL7 and Skin
Spleen Antibodies	>10 publications with IL7 and Spleen
Liver Antibodies	>9 publications with IL7 and Liver
Brain Antibodies	>7 publications with IL7 and Brain
Intestine Antibodies	>6 publications with IL7 and Intestine
Kidney Antibodies	>3 publications with IL7 and Kidney