Leukemia Inhibitory Factor, Rat (LIF) Active Protein

Name: LIF Active Protein; Leukemia Inhibitory Factor, Rat; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS650372
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650372

Leukemia Inhibitory Factor (LIF) human, a 20kD protein containing 181aa residues, is a multifunctional gylcoprotein that induces macrophage differentiation and supresses the proliferation of the M1 myeloid cell line. Synonyms for LIF include:… More details

$1060 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Leukemia Inhibitory Factor, Rat (LIF), Active Protein

Full Product Name

Leukemia Inhibitory Factor, Recombinant, Rat (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI)

Product Gene Name

LIF active protein

Host

E Coli

Form/Format

Supplied as a liquid in PBS, 0.02% Tween-20. Dilute further in PBS, 1% BSA or 0.02% Tween-20 to a final concentration 1ug/ml.

Purity/Purification

Highly Purified
95% (SDS-PAGE). Endotoxin: 0.1ng/mg (LAL)

Preparation and Storage

May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in PBS, 1% BSA or 0.02% Tween 20.

Molecular Weight

19.7kD

Chromosome Location

Chromosome: 22; NC_000022.10 (30636442..30642796, complement). Location: 22q12.2

OMIM

159540

3D Structure

ModBase 3D Structure for P15018

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Activity

The biological activity of recombinant human LIF is measured by its ability to stimulate the differentiation of the M1 mouse myeloid leukemic cell line. The specific activity is 1x10e8 units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1ml agar cultures.

Product Categories/Family for LIF active protein

Growth Factors; Cytokines; Growth Factors-LIF

Other Notes

Small volumes of LIF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIF purchase

MBS650372 is an active protein targeting LIF. Also known as Leukemia Inhibitory Factor, Recombinant, Rat (LIF, Cholinergic Differentiation Factor, CDF, DIA, Differentiation-stimulating Factor, D Factor, HILDA, Melanoma-derived LPL Inhibitor, MLPLI). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for LIF. It may not necessarily be applicable to this product.

NCBI GI #

178415

NCBI GeneID

3976

UniProt Primary Accession #

P15018

UniProt Secondary Accession #

Q52LZ2; B2RCW7

UniProt Related Accession #

P15018

NCBI Official Full Name

leukemia inhibitory factor

NCBI Official Synonym Full Names

leukemia inhibitory factor (cholinergic differentiation factor)

NCBI Official Symbol

LIF

NCBI Official Synonym Symbols

CDF; DIA; HILDA

UniProt Protein Name

Leukemia inhibitory factor

UniProt Synonym Protein Names

Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor

Protein Family

Leukemia inhibitory factor

UniProt Gene Name

LIF

UniProt Synonym Gene Names

HILDA

UniProt Entry Name

LIF_HUMAN

NCBI Protein Information

leukemia inhibitory factor; MLPLI; D factor; OTTHUMP00000198698; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation stimulating factor; differentiation-stimulating factor; differentiation inhibitory activity

NCBI Summary for LIF

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq]

UniProt Comments for LIF

Function: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Subcellular location: Secreted.

Pharmaceutical use: In phase II clinical trial. The drug is being developed by Amrad to assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART).

Sequence similarities: Belongs to the LIF/OSM family.

Research Articles on LIF

1. The epigenetic up-regulation of the LIF gene likely play an important role in the development of breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LIF active protein

Reference Product	PubMed Publications
LIFR active protein	>429 publications with LIF and LIFR
IL6ST active protein	>221 publications with LIF and IL6ST

Pathways associated with LIF active protein

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies	MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
TGF Beta Signaling Pathway antibodies	TGF Beta Signaling Pathway Diagram
Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway antibodies	Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway Diagram

Diseases associated with LIF active protein

Disease Name	Pubmed Publications
Leukemia Antibodies	>2765 publications with LIF and Leukemia
Neoplasms Antibodies	>646 publications with LIF and Neoplasms
Leukemia, Myeloid Antibodies	>214 publications with LIF and Leukemia, Myeloid
Inflammation Antibodies	>213 publications with LIF and Inflammation
Carcinoma Antibodies	>174 publications with LIF and Carcinoma
Cardiovascular Diseases Antibodies	>164 publications with LIF and Cardiovascular Diseases
Female Urogenital Diseases Antibodies	>127 publications with LIF and Female Urogenital Diseases
Autoimmune Diseases Antibodies	>82 publications with LIF and Autoimmune Diseases
Leukemia, Myeloid, Acute Antibodies	>67 publications with LIF and Leukemia, Myeloid, Acute
Lung Diseases Antibodies	>59 publications with LIF and Lung Diseases

Organs/Tissues associated with LIF active protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>428 publications with LIF and Bone
Brain Antibodies	>394 publications with LIF and Brain
Embryonic Tissue Antibodies	>289 publications with LIF and Embryonic Tissue
Muscle Antibodies	>288 publications with LIF and Muscle
Uterus Antibodies	>286 publications with LIF and Uterus
Bone Marrow Antibodies	>222 publications with LIF and Bone Marrow
Skin Antibodies	>162 publications with LIF and Skin
Lung Antibodies	>123 publications with LIF and Lung
Placenta Antibodies	>120 publications with LIF and Placenta
Eye Antibodies	>93 publications with LIF and Eye