Epidermal Growth Factor, Pro (EGF) Active Protein

EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF- (1). These proteins contain EGF-like domains with three intramolecular disulfide bonds between conserved cysteines (2). EGF family members are synthesized as transmembrane preproproteins with varying numbers of EGF-like domains (3). The extracellular region of mouse pro-EGF contains eight LDL R class B repeats and nine EGF-like domains (4). Mature EGF is derived from the juxtamembrane EGF-like domain. EGF binds ErbB1 and induces the formation of homodimers or heterodimers containing ErbB2 (5). pro-EGF is most highly expressed in the submaxillary gland and kidney (6). In the kidney, the 190 kD preproprotein is cleaved by membrane-associated serine proteases, liberating the extracellular region which is subsequently processed into smaller fragments including the 6 kD mature EGF (7-10). The various cleavage products produced in the kidney also are present in urine (9-11). In the submaxillary gland, however, nearly all EGF is processed intracellularly and stored in secretory vesicles (6, 12). The soluble precursor binds ErbB1 and induces cellular proliferation, although it is significantly less potent than mature EGF (8, 9). In human thyroid carcinoma cells, a splice variant of pro-EGF with a deletion in the cytoplasmic domain induces increased proliferative activity relative to wild type pro-EGF (13). Within the extracellular region, mouse pro-EGF shares 79% aa sequence identity with rat pro-EGF and 67%-69% with human, canine, feline, and porcine pro-EGF.

Small volumes of EGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS650397 is an active protein targeting EGF. Also known as Epidermal Growth Factor, Pro-, Recombinant, Mouse (Pro-EGF, EGF) (BSA Free).