CD40 Ligand, Soluble (CD40) Active Protein

Recombinant human soluble CD40 ligand is a 16.3kD protein containing 149aa residues comprising the receptor binding TNF-like domain of CD40L.

CD40 is a member of the TNF receptor family. it is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ?immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261aa type II transmembrane protein, which contains a 22aa cytoplasmic domain, a 24aa transmembrane domain and a 215aa extracellular domain. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L. It is generated in vivo by an intracellular proteolytic processing of the full length CD40L.
CD40 Ligand (CD40L/TRAP, CD154) is a type II membrane protein. It effectuates the helper function of T cells on resting B cells. Recombinant human soluble CD40 Ligand (sCD40L/TRAP) is a 16.3kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
The CD40 antigen contains four homologous repeating cysteine-rich extracellular domains. It is a single chain glycoprotein. It is known to be a member of the tumor necrosis factor/nerve growth factor superfamily. It shows a significant homology to the Hodgkin's disease-associated antigen, CD30. CD40 is present on all B cells except plasma cells. It is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 is found on normal and neoplastic B cells, strongly expressed by interdigitating cells (IDC), basal epithelial cells, carcinomas, macrophages, some endothelial cells, and follicular dendritic cells. It is a pan-B marker, absent only from terminally differentiated plasma cells.
CD40 receptor is expressed on antigen-presenting cells such as B cells, macrophages, dendritic cells and thymic epithelial cells. CD40 mediates stimulation of cell growth, differentiation and death. It also plays a role in regulating transcription in B cells.
CD40 is predicted to function in the process of B cell selection in the germinal center. Studies demonstrated that it induces strong homotypic adhesions in resting B cells. Together with Interleukin-4 (IL-4) maintains the cell cycle of B blasts and promotes the switch to IgE secretion. Puri ed B cells cultured in the CD40 system in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. The ligand for CD40 is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion.

Small volumes of CD40 Ligand, soluble active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS650421 is an active protein targeting CD40 Ligand, soluble. Also known as CD40 Ligand, soluble, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, hCD40L, sCD40L, CD178, gp39, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Su. Host: Human, recombinant (E Coli).