TNFSF13B Active Protein – Cat# MBS650459

BAFF (TNFSF13B) Active Protein

Name: TNFSF13B Active Protein; BAFF; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650459
Price: 790 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650459

MBS650459 is an active protein targeting TNFSF13B. Also known as BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK). More details

$790 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BAFF (TNFSF13B), Active Protein

Full Product Name

BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)

Product Gene Name

TNFSF13B active protein

Form/Format

Lyophilized from 5 mM Tris, pH 8.0, plus 3 mM NaCl. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Purity/Purification

Highly Purified
~95% (SDS PAGE, HPLC)

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

17.0kD, 153 amino acid residues

Chromosome Location

Chromosome: 13; NC_000013.10 (108921977..108959365). Location: 13q32-q34

OMIM

603969

3D Structure

ModBase 3D Structure for Q9Y275

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

ED50=5-10ng/ml, determined by the dose dependent stimulation of IL-8 production by human PBMCs. Please note that this biological activity may vary with each PBMC donor. The biological activity was also determined in a mouse splenocyte survival assay. The ED50 for this assay is 0.5 - 2.0 µg/mL. BAFF is a member of the TNF ligand superfamily, which serves as a costimulator of B cell proliferation and function. This recombinant protein corresponds to the TNF-like portion of BAFF's extracellular domain.

Endotoxin Level

~0.1ng/ug

Product Categories/Family for TNFSF13B active protein

Growth Factors; Cytokines; Growth Factors-Apoptosis

Other Notes

Small volumes of TNFSF13B active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFSF13B. It may not necessarily be applicable to this product.

NCBI GI #

4585275

NCBI GeneID

10673

UniProt Primary Accession #

Q9Y275

UniProt Secondary Accession #

Q6FHD6; Q7Z5J2

UniProt Related Accession #

Q8IZI4; Q8IZI5; Q8IZI6; Q9Y275

NCBI Official Full Name

B-cell activating factor

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 13b

NCBI Official Symbol

TNFSF13B

NCBI Official Synonym Symbols

DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 13B

UniProt Synonym Protein Names

B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1

Protein Family

Tumor necrosis factor ligand superfamily

UniProt Gene Name

TNFSF13B

UniProt Synonym Gene Names

BAFF; BLYS; TALL1; TNFSF20; ZTNF4

UniProt Entry Name

TN13B_HUMAN

NCBI Protein Information

tumor necrosis factor ligand superfamily member 13B; delta BAFF; Delta4 BAFF; OTTHUMP00000018691; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20

NCBI Summary for TNFSF13B

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

UniProt Comments for TNFSF13B

Function: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Ref.15Isoform 2 seems to inhibit isoform 1 secretion and bioactivity

By similarity. Ref.15

Subunit structure: Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Ref.4

Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted.

Tissue specificity: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Ref.4

Induction: Up-regulated by exposure to IFNG/IFN-gamma. Down-regulated by phorbol myristate acetate/ionomycin treatment.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.Isoform 2 is not efficiently shed from the membrane unlike isoform 1

By similarity.N-glycosylated.

Sequence similarities: Belongs to the tumor necrosis factor family.

Research Articles on TNFSF13B

1. Data show negative correlations for BAFF concentration vs blood B cell percentage and serum CXCL13 concentration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TNFSF13B active protein

Reference Product	PubMed Publications
TNFRSF13C active protein	>99 publications with TNFSF13B and TNFRSF13C
TNFSF13 active protein	>73 publications with TNFSF13B and TNFSF13
TNFRSF13B active protein	>63 publications with TNFSF13B and TNFRSF13B
TNFRSF17 active protein	>55 publications with TNFSF13B and TNFRSF17

Pathways associated with TNFSF13B active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Intestinal Immune Network For IgA Production Pathway antibodies	Intestinal Immune Network For IgA Production Pathway Diagram
Intestinal Immune Network For IgA Production Pathway antibodies	Intestinal Immune Network For IgA Production Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram

Diseases associated with TNFSF13B active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>81 publications with TNFSF13B and Neoplasms
Arthritis, Rheumatoid Antibodies	>68 publications with TNFSF13B and Arthritis, Rheumatoid
Inflammation Antibodies	>56 publications with TNFSF13B and Inflammation
Hematologic Diseases Antibodies	>43 publications with TNFSF13B and Hematologic Diseases
Leukemia Antibodies	>30 publications with TNFSF13B and Leukemia
Nervous System Diseases Antibodies	>28 publications with TNFSF13B and Nervous System Diseases
Multiple Myeloma Antibodies	>20 publications with TNFSF13B and Multiple Myeloma
Leukemia, Lymphocytic, Chronic, B-Cell Antibodies	>15 publications with TNFSF13B and Leukemia, Lymphocytic, Chronic, B-Cell
Hemorrhage Antibodies	>7 publications with TNFSF13B and Hemorrhage
Blood Coagulation Disorders Antibodies	>7 publications with TNFSF13B and Blood Coagulation Disorders

Organs/Tissues associated with TNFSF13B active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>468 publications with TNFSF13B and Blood
Bone Marrow Antibodies	>45 publications with TNFSF13B and Bone Marrow
Lymph Node Antibodies	>40 publications with TNFSF13B and Lymph Node
Kidney Antibodies	>25 publications with TNFSF13B and Kidney
Skin Antibodies	>12 publications with TNFSF13B and Skin
Brain Antibodies	>10 publications with TNFSF13B and Brain
Liver Antibodies	>9 publications with TNFSF13B and Liver
Pharynx Antibodies	>8 publications with TNFSF13B and Pharynx
Vascular Antibodies	>6 publications with TNFSF13B and Vascular
Thymus Antibodies	>6 publications with TNFSF13B and Thymus