Oncostatin M (OSM) Active Protein

Name: OSM Active Protein; Oncostatin M; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650576
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650576

Oncostatin M (OSM) is a member of the interleukin-6 family of cytokines. Of these cytokines, OSM is closely related structually, genetically and functionally to leukemia inhibitory factor. However, OSM-specific biological activities have been reported in… More details

$370 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Oncostatin M (OSM), Active Protein

Full Product Name

Oncostatin M, Recombinant, Human (OSM)

Product Gene Name

OSM active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Al a-Ile-Gly

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives.

Purity/Purification

Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

-20 degree C
Stability: Lyophilized Recombinant Human OSM although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution OSM should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight

26kD

Chromosome Location

Chromosome: 22; NC_000022.10 (30658818..30662840, complement). Location: 22q12.2

OMIM

165095

3D Structure

ModBase 3D Structure for P13725

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Human Oncostatin M is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5 x 105 IU/mg.

Dimers and Aggregates

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of recombinant OSM.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of OSM as a Reference Standard.

Solubility

It is recommended to reconstitute the lyophilized rHuOSM in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for OSM active protein

Growth Factors; Cytokines; Growth Factors-Oncostatin M

Other Notes

Small volumes of OSM active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OSM purchase

MBS650576 is an active protein targeting OSM. Also known as Oncostatin M, Recombinant, Human (OSM). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for OSM. It may not necessarily be applicable to this product.

NCBI GI #

10092621

NCBI GeneID

5008

NCBI Accession #

NP_065391.1

UniProt Primary Accession #

P13725

UniProt Secondary Accession #

Q6FHP8; Q9UCP6

UniProt Related Accession #

P13725

GenBank Nucleotide #

NM_020530.4

NCBI Official Full Name

oncostatin-M preproprotein

NCBI Official Synonym Full Names

oncostatin M

NCBI Official Symbol

OSM

NCBI Official Synonym Symbols

MGC20461

UniProt Protein Name

Oncostatin-M

Protein Family

Oncostatin

UniProt Gene Name

OSM

UniProt Entry Name

ONCM_HUMAN

NCBI Protein Information

oncostatin-M; OTTHUMP00000198700

NCBI Summary for OSM

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq]

UniProt Comments for OSM

Function: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration

By similarity. Ref.8 Ref.12

Subcellular location: Secreted.

Post-translational modification: Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.

Sequence similarities: Belongs to the LIF/OSM family.

Research Articles on OSM

1. a cytokine-triggered regulatory network for PCSK9 expression that is linked to JAKs and the ERK signaling pathway

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OSM active protein

Reference Product	PubMed Publications
LIFR active protein	>377 publications with OSM and LIFR
IL6ST active protein	>176 publications with OSM and IL6ST
OSMR active protein	>50 publications with OSM and OSMR

Pathways associated with OSM active protein

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with OSM active protein

Disease Name	Pubmed Publications
Leukemia Antibodies	>633 publications with OSM and Leukemia
Neoplasms Antibodies	>298 publications with OSM and Neoplasms
Inflammation Antibodies	>168 publications with OSM and Inflammation
Immune System Diseases Antibodies	>106 publications with OSM and Immune System Diseases
Cardiovascular Diseases Antibodies	>72 publications with OSM and Cardiovascular Diseases
Neuroectodermal Tumors Antibodies	>68 publications with OSM and Neuroectodermal Tumors
Skin Diseases Antibodies	>66 publications with OSM and Skin Diseases
Melanoma Antibodies	>58 publications with OSM and Melanoma
Vascular Diseases Antibodies	>58 publications with OSM and Vascular Diseases
Liver Diseases Antibodies	>56 publications with OSM and Liver Diseases

Organs/Tissues associated with OSM active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>402 publications with OSM and Blood
Brain Antibodies	>127 publications with OSM and Brain
Kidney Antibodies	>113 publications with OSM and Kidney
Lung Antibodies	>80 publications with OSM and Lung
Intestine Antibodies	>19 publications with OSM and Intestine
Thymus Antibodies	>17 publications with OSM and Thymus
Ovary Antibodies	>12 publications with OSM and Ovary
Uncharacterized Tissue Antibodies	>2 publications with OSM and Uncharacterized Tissue