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ZO-2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6506651

MBS6506651 is an antibody targeting ZO-2. Also known as ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2) (MaxLight 750). Reactive in Canine, Human, Mouse. Host: Rabbit. More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZO-2, Polyclonal Antibody
Full Product Name
ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2) (MaxLight 750)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Canine, Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight750.
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Specific for the canine ZO-2 protein (~160kD). Does exhibit crossreactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to crossreact with the related ZO-1 protein. Species crossreactivity: human and mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Synthetic peptide derived from the central portion of the ZO-2 protein.
Conjugate
MaxLight750
Positive Control
HeLa and canine MDCK cell lysates and mouse liver homogenates.
Note
Preservative Free
Applications Tested/Suitable for anti-ZO-2 antibody
Western Blot (WB), FLISA, Immunohistochemistry (IHC)
Application Notes for anti-ZO-2 antibody
WB: 1-4ug/ml
FLISA: 0.1-1ug/ml
Applications are based on unconjugated antibody.

Product References and Citations for anti-ZO-2 antibody

1. Anderson, J.M., et al. Curr. Opin. Cell. Biol. 5:772-778 (1993). 2. Gumbiner, B., et al. Am. J. Physiol. 123:1631-1633 (1993). 3. Stevenson, B.R., et al. J. Cell. Biol. 103:755-766 (1986). 4. Anderson, J.M., et al. J. Cell. Biol. 106:141-1149 (1988). 5. Itoh, M., et al. J. Cell. Biol. 115:1449-1462 (1993). 6. Gumbiner, B., et al. Proc. Natl. Acad. Sci. USA 88:3460-3464 (1991). 7. Citi, S., et al. Nature 33:272-276 (1988). 8. Zhong, Y., et al. J. Cell. Biol. 120:477-483 (1993). 9. Keon, B. H., et al. J. Cell. Biol. 134:1003-1018 (1996). 10. Furuse, M., et al. J. Cell Biol. 123:1777-1788 (1993). 11. Beatch, M., et al. J. Biol. Chem. 271:25723-25726 (1996). 12. Kim, S.K., et al. Curr. Opin. Cell. Biol. 7: 641-649 (1995). 13. Jesaitis, L.A., et al. J. Cell Biol. 124:949-961 (1994). 14. Woods, D.F., et al. Cell 66:451-464 (1991). 15. Ruff, P., et al. Proc. Natl. Acad. Sci. USA 88:6595-6599 (1991). 16. Cho, K., et al. Neuron 9:929-942 (1992). 17. Kistner, U., et al. J. Biol. Chem. 268:4580-4583 (1993). 18. Duclos, F., et al. Hum. Mol. Genet. 3:909-914 (1994).

Other Notes

Small volumes of anti-ZO-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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