Data show performance of human MCP-1 compared to a leading competitor's protein in chemotaxis of THP-1 cells. Data show no loss in protein bioactivity after lyophilization.

MCAF Active Protein

Name: MCAF Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650669
Price: 1165 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650669

MBS650669 is an active protein targeting MCAF. Also known as MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor). More details

$1165 / 0.025 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MCAF, Active Protein

Full Product Name

MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)

Form/Format

Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-0.5mg/ml.

Purity/Purification

Highly Purified
~98% (SDS-PAGE)

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

8kD

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

ED50=3.0-10ng/ml. The biological activity was determined by measuring dose dependent chemotaxis with human THP-1 cells.

Endotoxin Level

~0.1ng/ug

Product Categories/Family for MCAF active protein

Growth Factors; Cytokines; Growth Factors-MCAF

Other Notes

Small volumes of MCAF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCAF. It may not necessarily be applicable to this product.

NCBI GI #

29611342

NCBI Official Full Name

MBD1-containing chromatin associated factor

NCBI Summary for MCAF

ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010]

UniProt Comments for MCAF

ATF7IP: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells. Belongs to the MCAF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; ATPase activity; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of transcriptional preinitiation complex assembly

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MCAF active protein

Reference Product	PubMed Publications
FLNA active protein	>12 publications with MCAF and FLNA

Pathways associated with MCAF active protein

Products by Pathway	Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram

Organs/Tissues associated with MCAF active protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with MCAF and Bone
Connective Tissue Antibodies	>4 publications with MCAF and Connective Tissue
Heart Antibodies	>3 publications with MCAF and Heart
Muscle Antibodies	>3 publications with MCAF and Muscle
Brain Antibodies	>3 publications with MCAF and Brain
Vascular Antibodies	>2 publications with MCAF and Vascular
Embryonic Tissue Antibodies	>2 publications with MCAF and Embryonic Tissue
Ear Antibodies	>1 publications with MCAF and Ear
Kidney Antibodies	>1 publications with MCAF and Kidney
Umbilical Cord Antibodies	>1 publications with MCAF and Umbilical Cord