Western Blot analysis of extracts from primary human CD4+ T cells and various cell lines using MBS6506894 (upper) or B-Actin Rabbit mAb (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MBS6506894.
Immunohistochemical analysis of paraffin-embedded cell pellets, primary CD4+ T cells (left) and HT-29 cells (right), using MBS6506894 T cells were purified from human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml).
Immunohistochemical analysis of paraffin-embedded human tonsil using MBS6506894.
Flow cytometric analysis of Jurkat cells (blue) and primary CD4+ T cells (green) using MBS6506894. Controls were run on Jurkat cells (orange) and primary CD4+ T cells (red) using concentration-matched Rabbit mAb IgG Isotype Control. Anti-rabbit IgG (H+L), F(ab’)2 fragment was used as a secondary antibody. CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of Human Interleukin-2 (hIL-2) (6.7ng/ml)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TIM-3 Rabbit mAb. Note staining of alveolar macrophages.
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing full-length human Myc/DDK- tagged TIM-3 (hTIM-3-Myc/DDK; +), using TIM-3 Rabbit mAb.
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