Rat Anti-ErbB2 Monoclonal Antibody – Cat# MBS6507451

Flow Cytometry analysis of ErbB2 in 3T3-LI mouse cell line using MBS6507451 or isotype control antibody followed by Phycoerythrin-conjugated rat IgG secondary antibody.

ErbB2 Antibody – Rat Monoclonal

Name: ErbB2 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6507451
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6507451

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a 185kD type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in… More details

$1060 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340, Kiaa3023, Neu), Monoclonal Antibody

Full Product Name

ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340, Kiaa3023, Neu)

Product Gene Name

anti-ErbB2 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

12K88

Host

Rat

Species Reactivity

Mouse

Form/Format

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purity/Purification

Affinity Purified

Concentration

~0.5mg/ml (after reconstitution) (lot specific)

Specificity

Recognizes mouse ErbB2. <5% crosseactivity with recombinant human ErbB2, recombinant mouse ErbB3, recombinant mouse ErbB4 and recombinant mouse EGF R.

Preparation and Storage

Lyophilized and reconstituted products are stable for 12 months at -20 degree C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

138,579 Da

3D Structure

ModBase 3D Structure for P70424

Immunogen

Recombinant protein corresponding to Thr23-Thr653 of mouse ErbB2 expressed in NS0 cells

Product Categories/Family for anti-ErbB2 antibody

Antibodies-Primary; Antibodies-Oncoproteins

Applications Tested/Suitable for anti-ErbB2 antibody

ELISA (EIA), Flow Cytometry (FC/FACS)

Application Notes for anti-ErbB2 antibody

Flow Cytometry: 2.5ug labels 10e6 cells using 3T3-L-1 mouse embryonic fibroblast adipose-like cell line

Other Notes

Small volumes of anti-ErbB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ErbB2 purchase

MBS6507451 is an antibody targeting ErbB2. Also known as ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, CD340, Kiaa3023, Neu). Reactive in Mouse. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for ErbB2. It may not necessarily be applicable to this product.

NCBI GI #

54873610

NCBI GeneID

13866

NCBI Accession #

NP_001003817.1

UniProt Primary Accession #

P70424

UniProt Secondary Accession #

Q61525; Q6ZPE0

UniProt Related Accession #

P70424

GenBank Nucleotide #

NM_001003817.1

NCBI Official Full Name

receptor tyrosine-protein kinase erbB-2

NCBI Official Synonym Full Names

erb-b2 receptor tyrosine kinase 2

NCBI Official Symbol

Erbb2

NCBI Official Synonym Symbols

Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023

UniProt Protein Name

Receptor tyrosine-protein kinase erbB-2

UniProt Synonym Protein Names

Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; CD_antigen: CD340

Protein Family

Receptor tyrosine-protein kinase

UniProt Gene Name

Erbb2

UniProt Synonym Gene Names

Kiaa3023; Neu

NCBI Protein Information

receptor tyrosine-protein kinase erbB-2

UniProt Comments for ErbB2

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization ().

Research Articles on ErbB2

1. that myocardial ErbB2 and beta-adrenergic receptors signalling are linked in a feedback loop with beta-adrenergic receptors activation leading to increased ErbB2 expression and activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ErbB2 antibody

Reference Product	PubMed Publications
Egfr antibody	>3473 publications with ErbB2 and Egfr
Erbb3 antibody	>957 publications with ErbB2 and Erbb3
Src antibody	>469 publications with ErbB2 and Src
Ctnnb1 antibody	>219 publications with ErbB2 and Ctnnb1
Nrg1 antibody	>217 publications with ErbB2 and Nrg1
Stat3 antibody	>135 publications with ErbB2 and Stat3
Grb2 antibody	>111 publications with ErbB2 and Grb2
Erbb2ip antibody	>50 publications with ErbB2 and Erbb2ip
Shc1 antibody	>37 publications with ErbB2 and Shc1
Hsp90aa1 antibody	>3 publications with ErbB2 and Hsp90aa1

Pathways associated with anti-ErbB2 antibody

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Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Breast Cancer Pathway antibodies	Breast Cancer Pathway Diagram
Breast Cancer Pathway antibodies	Breast Cancer Pathway Diagram

Diseases associated with anti-ErbB2 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>14771 publications with ErbB2 and Breast Neoplasms
Adenocarcinoma Antibodies	>4113 publications with ErbB2 and Adenocarcinoma
Neoplasm Metastasis Antibodies	>3471 publications with ErbB2 and Neoplasm Metastasis
Recurrence Antibodies	>2120 publications with ErbB2 and Recurrence
Disease Progression Antibodies	>1969 publications with ErbB2 and Disease Progression
Neoplasms, Experimental Antibodies	>1911 publications with ErbB2 and Neoplasms, Experimental
Gastrointestinal Neoplasms Antibodies	>1637 publications with ErbB2 and Gastrointestinal Neoplasms
Lymphatic Metastasis Antibodies	>1565 publications with ErbB2 and Lymphatic Metastasis
Carcinogenesis Antibodies	>1468 publications with ErbB2 and Carcinogenesis
Lung Neoplasms Antibodies	>1412 publications with ErbB2 and Lung Neoplasms

Organs/Tissues associated with anti-ErbB2 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1656 publications with ErbB2 and Lung
Stomach Antibodies	>1001 publications with ErbB2 and Stomach
Brain Antibodies	>809 publications with ErbB2 and Brain
Liver Antibodies	>704 publications with ErbB2 and Liver
Bone Antibodies	>555 publications with ErbB2 and Bone
Heart Antibodies	>518 publications with ErbB2 and Heart
Mammary Gland Antibodies	>513 publications with ErbB2 and Mammary Gland
Skin Antibodies	>349 publications with ErbB2 and Skin
Kidney Antibodies	>246 publications with ErbB2 and Kidney
Ovary Antibodies	>203 publications with ErbB2 and Ovary