Ciliary Neurotrophic Factor (CNTF) Active Protein

Name: CNTF Active Protein; Ciliary Neurotrophic Factor; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650758
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650758

A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does… More details

$530 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ciliary Neurotrophic Factor (CNTF), Active Protein

Full Product Name

Ciliary Neurotrophic Factor, Recombinant, Human (CNTF)

Product Gene Name

CNTF active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ph e-Thr-Glu.

Host

E Coli

Form/Format

Supplied as a lyophilized powder in 5mM sodium phosphate buffer, pH 7.5, 5mM sodium chloride.

Purity/Purification

Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

22,931 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (58390146..58393206). Location: 11q12.2

OMIM

118945

3D Structure

ModBase 3D Structure for P26441

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Recombinant Human Ciliary Neurotrophic Factor is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5 x 10 IU/mg.

Dimers and Aggregates

1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug)

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard.

Reconstitution

Recommended to reconstitute the lyophilized CNTF in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for CNTF active protein

Growth Factors; Cytokines; Growth Factors-CNTF

Other Notes

Small volumes of CNTF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CNTF purchase

MBS650758 is an active protein targeting CNTF. Also known as Ciliary Neurotrophic Factor, Recombinant, Human (CNTF). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CNTF. It may not necessarily be applicable to this product.

NCBI GI #

4758020

NCBI GeneID

1270

NCBI Accession #

NP_000605.1

UniProt Primary Accession #

P26441

UniProt Secondary Accession #

B2RAB2

UniProt Related Accession #

P26441

GenBank Nucleotide #

NM_000614.3

NCBI Official Full Name

ciliary neurotrophic factor

NCBI Official Synonym Full Names

ciliary neurotrophic factor

NCBI Official Symbol

CNTF

NCBI Official Synonym Symbols

HCNTF

UniProt Protein Name

Ciliary neurotrophic factor

Protein Family

Ciliary neurotrophic factor

UniProt Gene Name

CNTF

UniProt Entry Name

CNTF_HUMAN

NCBI Protein Information

ciliary neurotrophic factor; OTTHUMP00000174731

NCBI Summary for CNTF

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq]

UniProt Comments for CNTF

Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Subunit structure: Homodimer.

Subcellular location: Cytoplasm.

Tissue specificity: Nervous system.

Sequence similarities: Belongs to the CNTF family.

Research Articles on CNTF

1. Data show that variants in CNTF were significantly associated with a lower age at onset of the eating disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CNTF active protein

Reference Product	PubMed Publications
LIFR active protein	>124 publications with CNTF and LIFR
IL6ST active protein	>107 publications with CNTF and IL6ST
CNTFR active protein	>100 publications with CNTF and CNTFR
APOE active protein	>4 publications with CNTF and APOE
IL6R active protein	>1 publications with CNTF and IL6R

Pathways associated with CNTF active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with CNTF active protein

Disease Name	Pubmed Publications
Leukemia Antibodies	>493 publications with CNTF and Leukemia
Nerve Degeneration Antibodies	>178 publications with CNTF and Nerve Degeneration
Neoplasms Antibodies	>144 publications with CNTF and Neoplasms
Spinal Cord Diseases Antibodies	>141 publications with CNTF and Spinal Cord Diseases
Neuroblastoma Antibodies	>87 publications with CNTF and Neuroblastoma
Neuroectodermal Tumors Antibodies	>87 publications with CNTF and Neuroectodermal Tumors
Drug Toxicity Antibodies	>82 publications with CNTF and Drug Toxicity
Inflammation Antibodies	>82 publications with CNTF and Inflammation
Necrosis Antibodies	>78 publications with CNTF and Necrosis
Obesity Antibodies	>72 publications with CNTF and Obesity

Organs/Tissues associated with CNTF active protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>1688 publications with CNTF and Nerve
Brain Antibodies	>1105 publications with CNTF and Brain
Embryonic Tissue Antibodies	>327 publications with CNTF and Embryonic Tissue
Ganglia Antibodies	>318 publications with CNTF and Ganglia
Eye Antibodies	>312 publications with CNTF and Eye
Muscle Antibodies	>289 publications with CNTF and Muscle
Spinal Cord Antibodies	>256 publications with CNTF and Spinal Cord
Blood Antibodies	>175 publications with CNTF and Blood
Bone Antibodies	>90 publications with CNTF and Bone
Liver Antibodies	>59 publications with CNTF and Liver