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Interleukin 10 (IL10) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650777

Recombinant Interleukin-10 comprises a 160 amino acid fragment (19-178) corresponding to the mature Interleukin-10 protein and is expressed in E. coli with an amino-terminal hexahistidine tag.Interleukin-10 is a multifunctional cytokine produced by a variety… More details

$590 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 10 (IL10), Recombinant Protein
Full Product Name
Interleukin 10, Recombinant, Human
Product Gene Name
Host
Human, Recombinant, E Coli
Form/Format
Supplied as a liquid.
Purity/Purification
Highly Purified
Purified. 95% by SDS-PAGE.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
20,517 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (206940948..206945839, complement). Location: 1q31-q32
OMIM
124092
3D Structure
ModBase 3D Structure for P22301
Applications Tested/Suitable for IL10 recombinant protein
ELISA (EL/EIA)
Application Notes for IL10 recombinant protein
Suitable for use in ELISA., , Recommended Dilution:, Optimal dilutions to be determined by the researcher.

SDS-Page of IL10 recombinant protein

>95% by SDS-PAGE of I8432-13S
IL10 Recombinant Protein – Cat# MBS650777

Other Notes

Small volumes of IL10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL10 purchase

MBS650777 is a recombinant protein targeting IL10. Also known as Interleukin 10, Recombinant, Human. Host: Human, Recombinant, E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
IL10
NCBI Official Synonym Symbols
CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451
UniProt Protein Name
Interleukin-10
UniProt Synonym Protein Names
Cytokine synthesis inhibitory factor
Protein Family
UniProt Gene Name
IL10
UniProt Entry Name
IL10_HUMAN
NCBI Protein Information
interleukin-10; OTTHUMP00000034567; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor

NCBI Summary for IL10

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
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UniProt Comments for IL10

Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Sequence similarities: Belongs to the IL-10 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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