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Interferon Regulatory Factor 3 (IRF3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6507784

Interferon regulatory factor 3 (IRF3) is a 50 kD, 427aa, member of the IRF family of proteins. Human IRF3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptors, TLR3 and TLR4. Viral entry… More details

$1075 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interferon Regulatory Factor 3 (IRF3), Polyclonal Antibody
Full Product Name
Interferon Regulatory Factor 3 (IRF3)
Product Gene Name
anti-IRF3 antibody
Product Synonym Names
Interferon Regulatory Factor 3
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity/Purification
Affinity Purified
Concentration
~0.2mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human IRF3. In direct ELISAs, ~20% cross-reactivity with recombinant mouse IRF3 is observed.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
47,219 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (50162826..50169132, complement). Location: 19q13.3-q13.4
OMIM
603734
3D Structure
ModBase 3D Structure for Q14653
Immunogen
Recombinant protein corresponding to Val206-Ser427 from human IRF3, expressed in E Coli
Species Sequence Homology
mouse (76%) and porcine (83%)

Product Categories/Family for anti-IRF3 antibody

Antibodies-Primary; Antibodies-Growth Factors, Cytokines
Applications Tested/Suitable for anti-IRF3 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunochemistry (IC), Western Blot (WB), Knockout (KO) Validated
Application Notes for anti-IRF3 antibody
Western Blot: 1ug/ml (w/ appropriate secondary reagents). Detection limit is ~10ng/lane (non-reducing) and ~1ng/lane (reducing).
Flow Cytometry: 50ug/ml and add 10ul of the diluted solution to 1-2.5x10e5 HeLa cells in a total reaction volume >=200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat IgG (FITC)
Immunocytochemistry: 10ug/ml detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4 degree C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA.
ELISA: 0.5-1.0ug/ml (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well.

Testing Data of anti-IRF3 antibody

Daudi human Burkitt's lymphoma cell line was stained with MBS6507784 (filled histogram) or isotype control antibody (open histogram), followed by Goat IgG (PE). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Goat Anti-IRF3 Polyclonal Antibody – Cat# MBS6507784

Western Blot (WB) of anti-IRF3 antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1ug/ml of MBS6507784 followed by Goat IgG (HRP). A specific band was detected for IRF3 at ~50kD (as indicated). This experiment was conducted under reducing conditions.
Goat Anti-IRF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6507784

Testing Data of anti-IRF3 antibody

IRF3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using MBS6507784 at 15ug/ml for 3 hours at room temperature. Cells were stained using the Anti-Goat IgG Secondary (NorthernLights™ 557) (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Goat Anti-IRF3 Polyclonal Antibody, Testing Data – Cat# MBS6507784

Other Notes

Small volumes of anti-IRF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRF3 purchase

MBS6507784 is an antibody targeting IRF3. Also known as Interferon Regulatory Factor 3 (IRF3). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for IRF3. It may not necessarily be applicable to this product.

NCBI GI #
308199450
NCBI GeneID
3661
NCBI Accession #
NP_001184053.1
UniProt Primary Accession #
Q14653
UniProt Secondary Accession #
Q5FBY1; A8K7L2
UniProt Related Accession #
Q14653
GenBank Nucleotide #
NM_001197124.1
NCBI Official Full Name
interferon regulatory factor 3 isoform 4
NCBI Official Synonym Full Names
interferon regulatory factor 3
NCBI Official Symbol
IRF3
UniProt Protein Name
Interferon regulatory factor 3
Protein Family
Interferon regulatory factor
UniProt Gene Name
IRF3
UniProt Synonym Gene Names
IRF-3
UniProt Entry Name
IRF3_HUMAN
NCBI Protein Information
interferon regulatory factor 3

NCBI Summary for IRF3

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for IRF3

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: negative regulation of interferon-beta biosynthetic process; transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; negative regulation of defense response to virus by host; response to exogenous dsRNA; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; toll-like receptor signaling pathway; innate immune response; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; negative regulation of interferon type I production; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7
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Research Articles on IRF3

1. Ultraviolet radiation also upregulated mitogen-activated protein kinases and nuclear factor-kappaB/p65 expression via Toll-like receptor 2 and Toll-like receptor 4, and upregulated interferon regulatory factor-3 expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRF3 antibody

 Reference Product  PubMed Publications
 IRF7 antibody  >197 publications with IRF3 and IRF7
 TBK1 antibody  >166 publications with IRF3 and TBK1
 CXCL10 antibody  >43 publications with IRF3 and CXCL10
 IRF5 antibody  >34 publications with IRF3 and IRF5
 IKBKE antibody  >27 publications with IRF3 and IKBKE
 RELA antibody  >21 publications with IRF3 and RELA
 CREBBP antibody  >13 publications with IRF3 and CREBBP
 EP300 antibody  >6 publications with IRF3 and EP300
 GRIP1 antibody  >3 publications with IRF3 and GRIP1
 PIAS4 antibody  >2 publications with IRF3 and PIAS4

Pathways associated with anti-IRF3 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DAI Mediated Induction Of Type I IFNs Pathway antibodies  DAI Mediated Induction Of Type I IFNs Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-IRF3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >144 publications with IRF3 and Inflammation
 Necrosis Antibodies  >113 publications with IRF3 and Necrosis
 Death Antibodies  >50 publications with IRF3 and Death
 Neoplasms Antibodies  >48 publications with IRF3 and Neoplasms
 Liver Diseases Antibodies  >44 publications with IRF3 and Liver Diseases
 Cardiovascular Diseases Antibodies  >22 publications with IRF3 and Cardiovascular Diseases
 Carcinoma Antibodies  >15 publications with IRF3 and Carcinoma
 Kidney Diseases Antibodies  >14 publications with IRF3 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >9 publications with IRF3 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >7 publications with IRF3 and Drug Toxicity

Organs/Tissues associated with anti-IRF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >131 publications with IRF3 and Blood
 Liver Antibodies  >50 publications with IRF3 and Liver
 Bone Antibodies  >45 publications with IRF3 and Bone
 Kidney Antibodies  >37 publications with IRF3 and Kidney
 Lung Antibodies  >36 publications with IRF3 and Lung
 Skin Antibodies  >22 publications with IRF3 and Skin
 Brain Antibodies  >22 publications with IRF3 and Brain
 Spleen Antibodies  >21 publications with IRF3 and Spleen
 Vascular Antibodies  >17 publications with IRF3 and Vascular
 Intestine Antibodies  >10 publications with IRF3 and Intestine
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