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Interleukin 1 Beta (IL-1b) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6507788

Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues. More details

$1135 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 1 beta (IL-1b), Polyclonal Antibody
Full Product Name
Interleukin 1 beta (IL-1b)
Product Gene Name
Product Synonym Names
Interleukin 1 beta
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity/Purification
Affinity Purified
Concentration
~0.2mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human IL-1 beta. In Direct ELISAs, ~30?75% crossreactivity with recombinant mouse IL?1b, recombinant porcine IL-1b, recombinant rat IL-1b, recombinant canine IL-1b, recombinant equine IL-1b, recombinant cotton rat IL-1b, recombinant feline IL?1b, recombinant Rhesus monkey IL-1b and recombinant guinea pig IL-1b.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
17kDa (154aa), confirmed by MALDI-TOF.
OMIM
147720
3D Structure
ModBase 3D Structure for P01584
Immunogen
Recombinant protein corresponding to aa117-269 from human IL-1b expressed in E Coli (NP_000567).
Applications Tested/Suitable for anti-IL-1b antibody
ELISA (EIA), Immunochemistry (IC), Neutralizing (NEU), Western Blot (WB)
Application Notes for anti-IL-1b antibody
Western Blot: 0.1ug/ml using recombinant human IL-1b
Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood lymphocytes and immersion fixed human peripheral blood mononuclear cells treated with LPS and monensin.
Neutralization: 0.004-0.00ug/ml in the presence of 50pg/ml recombinant human IL-1b and 1.25ug/ml concanavalin A. Measured by its ability to neutralize IL 2011;1 b-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.

Other Notes

Small volumes of anti-IL-1b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-1b purchase

MBS6507788 is an antibody targeting IL-1b. Also known as Interleukin 1 beta (IL-1b). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-1b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-1 beta proprotein
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
IL1B
NCBI Official Synonym Symbols
IL-1; IL1F2; IL1-BETA
UniProt Protein Name
Interleukin-1 beta
UniProt Synonym Protein Names
Catabolin
UniProt Gene Name
IL1B
UniProt Synonym Gene Names
IL1F2; IL-1 beta
UniProt Entry Name
IL1B_HUMAN
NCBI Protein Information
interleukin-1 beta

NCBI Summary for IL-1b

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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