Interleukin 13 (IL13) Active Protein

Name: IL13 Active Protein; Interleukin 13; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS650797
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650797

Interleukin-13 (IL-13) is a multifunctional cytokine whose net principle action is to diminish inflammatory responses. Interleukin (IL)-13 is an important cytokine secreted from type 2 helper T lymphocytes. It is essential for modulating IgE synthesis by… More details

$370 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 13 (IL13), Active Protein

Full Product Name

Interleukin 13, Recombinant, Human (IL-13)

Product Gene Name

IL13 active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and found to be Ser-Pro-Gl y-Pro-Val.

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives.

Purity/Purification

Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

-20 degree C
Stability: Lyophilized Recombinant Human Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Human Interleukin-13 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight

12.5kD

Chromosome Location

Chromosome: 5; NC_000005.9 (131993865..131996801). Location: 5q31

OMIM

147683

3D Structure

ModBase 3D Structure for P35225

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Human IL-13 is fully biologically active when compared to standard. The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be < 1ng/ml, corresponding to a specific activity of >1 x 106.

Dimers and Aggregates

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of recombinant Human IL-13.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.

Solubility

It is recommended to reconstitute the lyophilized Human IL-13 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for IL13 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

Other Notes

Small volumes of IL13 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL13 purchase

MBS650797 is an active protein targeting IL13. Also known as Interleukin 13, Recombinant, Human (IL-13). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL13. It may not necessarily be applicable to this product.

NCBI GI #

26787978

NCBI GeneID

3596

NCBI Accession #

NP_002179.2

UniProt Primary Accession #

P35225

UniProt Secondary Accession #

O43644; Q4VB52; Q9UDC7

UniProt Related Accession #

P35225; Q4VB50; Q4VB51; Q4VB53

GenBank Nucleotide #

NM_002188.2

NCBI Official Full Name

interleukin-13

NCBI Official Synonym Full Names

interleukin 13

NCBI Official Symbol

IL13

NCBI Official Synonym Symbols

ALRH; BHR1; P600; IL-13; MGC116786; MGC116788; MGC116789

UniProt Protein Name

Interleukin-13

Protein Family

Interleukin

UniProt Gene Name

IL13

UniProt Synonym Gene Names

NC30

UniProt Entry Name

IL13_HUMAN

NCBI Protein Information

interleukin-13; allergic rhinitis; OTTHUMP00000066048; Bronchial hyperresponsiveness-1 (bronchial asthma)

NCBI Summary for IL13

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq]

UniProt Comments for IL13

Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.

Subunit structure: Interacts with IL13RA2.

Subcellular location: Secreted.

Involvement in disease: Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH) [

MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

Sequence similarities: Belongs to the IL-4/IL-13 family.

Caution: It is uncertain whether Met-1 or Met-15 is the initiator.

Sequence caution: The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Research Articles on IL13

1. Epigenetic regulation of the IL-13-induced human eotaxin-3 gene by CREB-binding protein-mediated histone 3 acetylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL13 active protein

Reference Product	PubMed Publications
IL13RA1 active protein	>272 publications with IL13 and IL13RA1
IL4R active protein	>41 publications with IL13 and IL4R
IL13RA2 active protein	>10 publications with IL13 and IL13RA2
VCAM1 active protein	>4 publications with IL13 and VCAM1

Pathways associated with IL13 active protein

Products by Pathway	Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
IL12 Signaling Mediated By STAT4 Pathway antibodies	IL12 Signaling Mediated By STAT4 Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with IL13 active protein

Disease Name	Pubmed Publications
Hypersensitivity Antibodies	>984 publications with IL13 and Hypersensitivity
Inflammation Antibodies	>914 publications with IL13 and Inflammation
Asthma Antibodies	>895 publications with IL13 and Asthma
Hypersensitivity, Immediate Antibodies	>849 publications with IL13 and Hypersensitivity, Immediate
Lung Diseases Antibodies	>839 publications with IL13 and Lung Diseases
Respiratory Hypersensitivity Antibodies	>711 publications with IL13 and Respiratory Hypersensitivity
Neoplasms Antibodies	>382 publications with IL13 and Neoplasms
Eosinophilia Antibodies	>235 publications with IL13 and Eosinophilia
Fibrosis Antibodies	>228 publications with IL13 and Fibrosis
Bronchial Hyperreactivity Antibodies	>178 publications with IL13 and Bronchial Hyperreactivity

Organs/Tissues associated with IL13 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>137 publications with IL13 and Brain
Bone Marrow Antibodies	>109 publications with IL13 and Bone Marrow
Mixed Antibodies	>58 publications with IL13 and Mixed
Testis Antibodies	>5 publications with IL13 and Testis