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TUBA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6508400

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and… More details

$1055 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1), Monoclonal Antibody
Full Product Name
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Product Gene Name
Product Synonym Names
Tubulin, alpha
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.N.593
Host
Mouse
Species Reactivity
Bovine, Chicken, Frog, Guinea Pig, Human, Mouse, Porcine, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T9155-50X.
Purity/Purification
Affinity Purified
Concentration
~0.2mg/ml (lot specific)
Specificity
Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps (1). Does not block microtubule assembly (1). Does not inhibit polymerization or depolymerization of platelet tubulin in vitro (3). Blocks (70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species Crossreactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Native chick brain microtubules
Epitope
aa426-450
Cellular Localization
Cytoplasmic
Positive Control
LS174T, MAD109 cells. Skin or lung
Applications Tested/Suitable for anti-TUBA1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TUBA1 antibody
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:400 for 20 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

Western Blot (WB) of anti-TUBA1 antibody

Western Blot using MBS6508400.
Mouse Anti-TUBA1 Monoclonal Antibody – Cat# MBS6508400

Immunohistochemsitry (IHC) of anti-TUBA1 antibody

Formalin-fixed, paraffin-embedded human lung stained with MBS6508400 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of ciliated epithelium of bronchioles.
Mouse Anti-TUBA1 Monoclonal Antibody, Immunohistochemsitry (IHC) – Cat# MBS6508400

Immunohistochemistry (IHC) of anti-TUBA1 antibody

Formalin-fixed, paraffin-embedded rat lung stained with MBS6508400 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of ciliated epithelium of bronchioles.
Mouse Anti-TUBA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6508400

Other Notes

Small volumes of anti-TUBA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBA1 purchase

MBS6508400 is an antibody targeting TUBA1. Also known as Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1). Reactive in Bovine, Chicken, Frog, Guinea Pig, Human, Mouse, Porcine, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBA1. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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