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25-Hydroxy Vitamin D3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6508497

MBS6508497 is an antibody targeting 25-Hydroxy Vitamin D3. Also known as Mouse Anti-25-Hydroxy Vitamin D3. Host: Mouse. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
25-Hydroxy Vitamin D3, Monoclonal Antibody
Full Product Name
Mouse Anti-25-Hydroxy Vitamin D3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14D3
Host
Mouse
Form/Format
Supplied as a powder lyophilized from PBS, pH 7.4, 2% BSA.
Purity/Purification
Affinity Purified. Purified by Protein G affinity chromatography.
Concentration
~0.2mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes 25(OH)D3 (100%).
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
25(OH)D3 3-Hemisuccinate-BSA
Crossreactivity
24,25(OH)2D3: >=100%, 25(OH)D2: 68%, D2: 0.3%, D3: <0.1%
Reconstitution
Reconstitute with 5ml sterile ddH2O
Applications Tested/Suitable for anti-25-Hydroxy Vitamin D3 antibody
ELISA (EIA)
ELISA: 1:15,000-1:50,000

Product References and Citations for anti-25-Hydroxy Vitamin D3 antibody

1. "Nomenclature of Vitamin D. Recommendations 1981. IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN)" reproduced at the Queen Mary, University of London website. Retrieved 21 March 2010.
2. Holick, MF; Deluca, HF; Avioli, LV (1972). "Isolation and identification of 25-hydroxycholecalciferol from human plasma". Archives of Internal Medicine 129 (1): 56–61. doi:10.1001/archinte.1972.00320010060005. PMID 4332591.
3. Am J Clin Nutr 2008;87:1738–42 PMID 18541563
4. Bender, David A.; Mayes, Peter A (2006). "Micronutrients: Vitamins & Minerals". In Victor W. Rodwell; Murray, Robert F.; Harper, Harold W.; Granner, Darryl K.; Mayes, Peter A. Harper's Illustrated Biochemistry. New York: Lange/McGraw-Hill. pp. 492–3. ISBN 0-07-146197-3.Retrieved December 10, 2008

Other Notes

Small volumes of anti-25-Hydroxy Vitamin D3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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