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Erythropoietin, Beta (EPO) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650859

Recombinant Human Erythropoietin-beta is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21kD and apparent glycosylated molecular… More details

$1095 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Erythropoietin, beta (Epo), Active Protein
Full Product Name
Erythropoietin, beta, Recombinant, Human (EPO)
Product Gene Name
Host
CHO cells
Form/Format
Supplied as a lyophlilzed powder in sodium phosphate dibasic, sodium phosphate monobasic buffer, 60% sodium chloride, 0.6% calcium chloride and 0.004% Tween 20.
Purity/Purification
Highly Purified
98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
21,286 Da
Chromosome Location
Chromosome: 12; NC_005111.3 (24291481..24294921). Location: 12q12-q13
3D Structure
ModBase 3D Structure for P29676
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Erythropoietin is fully biologically active when compared to standard. The Specific Activity was measured by Normocyth-aemic mice and was found to be 1.2x10e5IU/mg.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of EPO-b.
Reconstitution
Reconstitute the lyophilized EPO-b in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Other Notes

Small volumes of Epo active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Epo purchase

MBS650859 is an active protein targeting Epo. Also known as Erythropoietin, beta, Recombinant, Human (EPO). Host: CHO cells.

Additional Details

NCBI/Uniprot data below describe general gene information for Epo. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
erythropoietin
NCBI Official Synonym Full Names
erythropoietin
NCBI Official Symbol
Epo
UniProt Protein Name
Erythropoietin
Protein Family
UniProt Gene Name
Epo
UniProt Entry Name
EPO_RAT
NCBI Protein Information
erythropoietin

NCBI Summary for Epo

acts as a regulator of erythropoiesis; may facilitate oxygen delivery in response to hypoxia [RGD, Feb 2006]

UniProt Comments for Epo

Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Subcellular location: Secreted.

Tissue specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Sequence similarities: Belongs to the EPO/TPO family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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