Granulocyte Macrophage-Colony Stimulating Factor, Rat (CSF2) Active Protein

Name: CSF2 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS650896
Price: 790 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650896

The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterized by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the… More details

$790 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Granulocyte Macrophage-Colony Stimulating Factor, Rat (CSF2), Active Protein

Full Product Name

Granulocyte Macrophage-Colony Stimulating Factor, Recombinant, Rat (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Plur

Product Gene Name

CSF2 active protein

Product Synonym Names

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pr o-Thr-Arg.

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives. Reconstitute in sterile ddH20, 0.1% BSA or HSA to 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity/Purification

Highly Purified
95% by RP-HPLC or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH20, 0.1% BSA or HSA, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

14.59kD

Chromosome Location

Chromosome: 5; NC_000005.9 (131409485..131411859). Location: 5q31.1

OMIM

138960

3D Structure

ModBase 3D Structure for P04141

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of murine MC/9 cells is 0.001-0.01ng/ml.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Rat GM-CSF as a Reference Standard.

Product Categories/Family for CSF2 active protein

Growth Factors; Cytokines; Growth Factors-GM-CSF

Other Notes

Small volumes of CSF2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSF2 purchase

MBS650896 is an active protein targeting CSF2. Also known as Granulocyte Macrophage-Colony Stimulating Factor, Recombinant, Rat (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Plur. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF2. It may not necessarily be applicable to this product.

NCBI GI #

27437030

NCBI GeneID

1437

NCBI Accession #

NP_000749.2

UniProt Primary Accession #

P04141

UniProt Secondary Accession #

Q14CE8; Q2VPI8; Q8NFI6

UniProt Related Accession #

P04141; Q647J8

GenBank Nucleotide #

NM_000758.2

NCBI Official Full Name

granulocyte-macrophage colony-stimulating factor

NCBI Official Synonym Full Names

colony stimulating factor 2 (granulocyte-macrophage)

NCBI Official Symbol

CSF2

NCBI Official Synonym Symbols

GMCSF; MGC131935; MGC138897

UniProt Protein Name

Granulocyte-macrophage colony-stimulating factor

UniProt Synonym Protein Names

Colony-stimulating factor; CSF; Molgramostin; Sargramostim

Protein Family

Granulocyte-macrophage colony-stimulating factor

UniProt Gene Name

CSF2

UniProt Synonym Gene Names

GMCSF

UniProt Entry Name

CSF2_HUMAN

NCBI Protein Information

granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; OTTHUMP00000065962; granulocyte-macrophage colony stimulating factor

NCBI Summary for CSF2

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq]

UniProt Comments for CSF2

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.

Research Articles on CSF2

1. The levels of EPO, SCF and GM-CSF in chronic aplastic anemia patients were obviously lower than those in the healthy individuals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CSF2 active protein

Reference Product	PubMed Publications
CSF2RB active protein	>6 publications with CSF2 and CSF2RB
CSF2RA active protein	>4 publications with CSF2 and CSF2RA
KITLG active protein	>2 publications with CSF2 and KITLG
IL3RA active protein	>2 publications with CSF2 and IL3RA
SDC2 active protein	>1 publications with CSF2 and SDC2

Pathways associated with CSF2 active protein

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with CSF2 active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>17 publications with CSF2 and Necrosis
Inflammation Antibodies	>14 publications with CSF2 and Inflammation
Neoplasms Antibodies	>10 publications with CSF2 and Neoplasms
Immune System Diseases Antibodies	>9 publications with CSF2 and Immune System Diseases
Respiratory Tract Diseases Antibodies	>6 publications with CSF2 and Respiratory Tract Diseases
Bacterial Infections Antibodies	>6 publications with CSF2 and Bacterial Infections
Leukemia Antibodies	>6 publications with CSF2 and Leukemia
Hematologic Diseases Antibodies	>6 publications with CSF2 and Hematologic Diseases
Lung Diseases Antibodies	>5 publications with CSF2 and Lung Diseases
Bone Marrow Diseases Antibodies	>5 publications with CSF2 and Bone Marrow Diseases

Organs/Tissues associated with CSF2 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>22 publications with CSF2 and Blood
Bone Antibodies	>6 publications with CSF2 and Bone
Lung Antibodies	>3 publications with CSF2 and Lung