Keratinocyte Growth Factor 2 (FGF10) Active Protein

Name: FGF10 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS650907
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650907

KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. Recombinant Human KGF-2 (FGF-10) is a single,… More details

$370 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Keratinocyte Growth Factor 2 (FGF10), Active Protein

Full Product Name

Keratinocyte Growth Factor 2, Recombinant, Human (KGF2)

Product Gene Name

FGF10 active protein

Host

E Coli

Form/Format

Supplied as a lyophilized powder in 5mM sodium phosphate, pH 7.4, 80mM sodium chloride.

Purity/Purification

Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

23,436 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (44305097..44388784, complement). Location: 5p13-p12

OMIM

149730

3D Structure

ModBase 3D Structure for O15520

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Recombinant Keratinocye Growth Factor-2 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent stimulation of FGF recpetors by BaF3 indicator cells (measured by 3H-thymidine uptake) is less then 0.5ng/ml corresponding to a specific activity of 2 x 10e6 Units/mg.

Dimers and Aggregates

1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of KGF-2.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.79 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of KGF-2 as a Reference Standard.

Reconstitution

Reconstitute the lyophilized KGF-2 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for FGF10 active protein

Growth Factors; Cytokines; Growth Factors-KGF

Other Notes

Small volumes of FGF10 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FGF10 purchase

MBS650907 is an active protein targeting FGF10. Also known as Keratinocyte Growth Factor 2, Recombinant, Human (KGF2). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for FGF10. It may not necessarily be applicable to this product.

NCBI GI #

2231295

NCBI GeneID

2255

UniProt Primary Accession #

O15520

UniProt Secondary Accession #

Q6FHT6; Q96P59

UniProt Related Accession #

O15520; Q6FHR3; Q8NFI9

NCBI Official Full Name

keratinocyte growth factor 2

NCBI Official Synonym Full Names

fibroblast growth factor 10

NCBI Official Symbol

FGF10

UniProt Protein Name

Fibroblast growth factor 10

UniProt Synonym Protein Names

Keratinocyte growth factor 2

Protein Family

Fibroblast growth factor

UniProt Gene Name

FGF10

UniProt Entry Name

FGF10_HUMAN

NCBI Protein Information

fibroblast growth factor 10; FGF-10; OTTHUMP00000161804; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria

NCBI Summary for FGF10

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq]

UniProt Comments for FGF10

Function: Could be a growth factor active in the process of wound healing. Acts as a mitogen in the lung. May act in a manner similar to FGF-7.

Subunit structure: Interacts with FGFBP1. Ref.7

Subcellular location: Secreted

Potential.

Involvement in disease: Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG) [

MIM:180920]. ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections. Ref.9Defects in FGF10 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [

MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed. Ref.10 Ref.11

Sequence similarities: Belongs to the heparin-binding growth factors family.

Research Articles on FGF10

1. There was no association among gene FGFR1 rs13317, p. E467K, p. M369I, p. S393S and gene FGF10 rs1448037 and nonsyndromic cleft lip with or without palate in Chinese population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FGF10 active protein

Reference Product	PubMed Publications
FGFR2 active protein	>132 publications with FGF10 and FGFR2
POMC active protein	>2 publications with FGF10 and POMC

Pathways associated with FGF10 active protein

Products by Pathway	Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram
FGFR Ligand Binding And Activation Pathway antibodies	FGFR Ligand Binding And Activation Pathway Diagram
FGFR1 Ligand Binding And Activation Pathway antibodies	FGFR1 Ligand Binding And Activation Pathway Diagram
FGFR1b Ligand Binding And Activation Pathway antibodies	FGFR1b Ligand Binding And Activation Pathway Diagram
FGFR2 Ligand Binding And Activation Pathway antibodies	FGFR2 Ligand Binding And Activation Pathway Diagram
FGFR2b Ligand Binding And Activation Pathway antibodies	FGFR2b Ligand Binding And Activation Pathway Diagram
FRS2-mediated Cascade Pathway antibodies	FRS2-mediated Cascade Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
IRS-mediated Signalling Pathway antibodies	IRS-mediated Signalling Pathway Diagram
IRS-related Events Pathway antibodies	IRS-related Events Pathway Diagram

Diseases associated with FGF10 active protein

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>83 publications with FGF10 and Congenital Abnormalities
Neoplasms Antibodies	>32 publications with FGF10 and Neoplasms
Craniofacial Abnormalities Antibodies	>26 publications with FGF10 and Craniofacial Abnormalities
Cleft Palate Antibodies	>19 publications with FGF10 and Cleft Palate
Limb Deformities, Congenital Antibodies	>18 publications with FGF10 and Limb Deformities, Congenital
Genital Diseases, Male Antibodies	>16 publications with FGF10 and Genital Diseases, Male
Carcinoma Antibodies	>15 publications with FGF10 and Carcinoma
Lung Diseases Antibodies	>14 publications with FGF10 and Lung Diseases
Jaw Abnormalities Antibodies	>14 publications with FGF10 and Jaw Abnormalities
Inflammation Antibodies	>13 publications with FGF10 and Inflammation

Organs/Tissues associated with FGF10 active protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>38 publications with FGF10 and Muscle
Brain Antibodies	>33 publications with FGF10 and Brain
Intestine Antibodies	>27 publications with FGF10 and Intestine
Kidney Antibodies	>20 publications with FGF10 and Kidney
Trachea Antibodies	>15 publications with FGF10 and Trachea
Uterus Antibodies	>7 publications with FGF10 and Uterus
Mixed Antibodies	>4 publications with FGF10 and Mixed