Macrophage Inflammatory Protein 1 Alpha, Rat (CCL3) Active Protein

Name: CCL3 Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS651047
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS651047

Recombinant Rat MIP-1a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. Recombinant Rat MIP-1a is purified by proprietary chromatographic techniques. More details

$370 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Macrophage Inflammatory Protein 1 alpha, Rat (CCL3), Active Protein

Full Product Name

Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)

Product Gene Name

CCL3 active protein

Sequence

The sequence of the first five N-terminal amino acids: Ala-Pro-Ty r-Gly-Ala.

Host

E Coli

Form/Format

Supplied as a lyophilized powder without additives.

Purity/Purification

Highly Purified
95% as determined by RP-HPLC, or SDS-PAGE.

Preparation and Storage

-20 degree C
Stability: Lyophilized MIP-1alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIP-1 alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight

7.85kD

Chromosome Location

Chromosome: 17; NC_000017.10 (34415602..34417506, complement). Location: 17q12

OMIM

182283

3D Structure

ModBase 3D Structure for P10147

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Recombinant Rat MIP-1a is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 alpha as a Reference Standard.

Product Categories/Family for CCL3 active protein

Growth Factors; Cytokines; Growth Factors-MIP

Other Notes

Small volumes of CCL3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL3 purchase

MBS651047 is an active protein targeting CCL3. Also known as Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL3. It may not necessarily be applicable to this product.

NCBI GI #

2905628

NCBI GeneID

6348

UniProt Primary Accession #

P10147

UniProt Related Accession #

P10147; Q14745

NCBI Official Full Name

macrophage inflammatory protein 1 alpha

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 3

NCBI Official Symbol

CCL3

NCBI Official Synonym Symbols

MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha

UniProt Protein Name

C-C motif chemokine 3

UniProt Synonym Protein Names

G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinCleaved into the following chain:MIP-1-alpha(4-69); Alternative name(s):; LD78-alpha(4-69)

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL3

UniProt Synonym Gene Names

G0S19-1; MIP1A; SCYA3

UniProt Entry Name

CCL3_HUMAN

NCBI Protein Information

C-C motif chemokine 3; SIS-beta; PAT 464.1; OTTHUMP00000163953; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)

NCBI Summary for CCL3

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.

UniProt Comments for CCL3

Function: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Ref.8

Subunit structure: Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69). Ref.11

Subcellular location: Secreted.

Induction: By TPA or PHA (TPA = 12-O-tetradecanoyl phorbol-13 acetate (tumor promoter); PHA = phytohemagglutinin (T-cell mitogen)).

Post-translational modification: N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Research Articles on CCL3

1. MIP-1alpha induces proliferation of nonasthmatic airway smooth muscle cells and decreases their rate of apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCL3 active protein

Reference Product	PubMed Publications
CCR5 active protein	>418 publications with CCL3 and CCR5
CCR1 active protein	>232 publications with CCL3 and CCR1
CCR3 active protein	>60 publications with CCL3 and CCR3
CCR4 active protein	>36 publications with CCL3 and CCR4
TGFB1 active protein	>28 publications with CCL3 and TGFB1
BCL6 active protein	>3 publications with CCL3 and BCL6
CEBPB active protein	>1 publications with CCL3 and CEBPB

Pathways associated with CCL3 active protein

Products by Pathway	Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
IL12-mediated Signaling Events Pathway antibodies	IL12-mediated Signaling Events Pathway Diagram

Diseases associated with CCL3 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>618 publications with CCL3 and Inflammation
Nervous System Diseases Antibodies	>194 publications with CCL3 and Nervous System Diseases
Lung Diseases Antibodies	>181 publications with CCL3 and Lung Diseases
HIV Infections Antibodies	>161 publications with CCL3 and HIV Infections
Cardiovascular Diseases Antibodies	>123 publications with CCL3 and Cardiovascular Diseases
Vascular Diseases Antibodies	>114 publications with CCL3 and Vascular Diseases
Skin Diseases Antibodies	>75 publications with CCL3 and Skin Diseases
Leukemia Antibodies	>73 publications with CCL3 and Leukemia
Arthritis, Rheumatoid Antibodies	>69 publications with CCL3 and Arthritis, Rheumatoid
Liver Diseases Antibodies	>65 publications with CCL3 and Liver Diseases

Organs/Tissues associated with CCL3 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1186 publications with CCL3 and Blood
Lung Antibodies	>245 publications with CCL3 and Lung
Brain Antibodies	>179 publications with CCL3 and Brain
Liver Antibodies	>159 publications with CCL3 and Liver
Kidney Antibodies	>60 publications with CCL3 and Kidney
Heart Antibodies	>56 publications with CCL3 and Heart
Eye Antibodies	>39 publications with CCL3 and Eye
Intestine Antibodies	>39 publications with CCL3 and Intestine
Uterus Antibodies	>17 publications with CCL3 and Uterus
Placenta Antibodies	>14 publications with CCL3 and Placenta