Lactobacilli MRS Broth, w/o Dextrose Culture Media

Western Blot analysis of human liver lysate (2Oug/lane) using MBS652659 (1:1000) .
Secondary: lgC (H+L) (HRP) goat anti-rabbit lgC, (H+L), (1:10,000) Predicted band size: 55kD Blocking/Dilution buffer: 50/o NFDMIBST.

Western Blot analysis of human liver lysate (20 ug/lane) using MBS 652659 (1:1000). Secondary: IgG (H+L) (HRP) goat anti- rabbit IgG, (H+L), (1:10,000)
Predicted band size: 55 kD
Blocking/ Dilution Buffer: 5% NFDM/ TBST.

Western Blot analysis using (MBS625659). Lane 1: Human brain lysate.
Lane 2 : Human liver lysate.
Lane 3. Mouse brain lysate.
Lysates / protein at 20 ug/lane.
Secondary: IgG (H+L) (HRP) goat-anti rabbit IgG (H+L, (1:10,000)

Overlay histogram showing Jurkat cells using MBS625659 (l :25) (green line). The cells were fixed with 2olo paraformaldehyde and then permeabilized with 90% methanol for '10 minutes. The cells were then icubated in 2olo BSA to block non-specific protein-protein interactions followed by MBS652659 (l :25) for 60 minutes at 37oC. The secondary antibody used was goat-anti-rabbit lgG, Dylight@ 4BB Conjugated Highly Cross-Adsorbed at l:200 for 40 minutes at RT. lsotype control antibody (blue line) was rabbit lgG 1 (l ug/lxl0e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.