Chick Fibroblast Basal Medium MCDB 201 Culture Media

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

≤1 EU/ml

Cell Culture Grade

White , homogenous, free flowing powder

5.6

Pale yellow, clear, complete

Dissolve 17.6g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Ammonium Metavanadate: 0.00001
Ammonium Molybdate•H2O: 0.000618
Calcium Chloride•2H20: 294.0
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.668
Magnesium Sulfate: 180.57
Manganese Sulfate: 0.000075
Nickel Chloride•6H2O: 0.0000012
Potassium Chloride: 223.65
Sodium Chloride: 7597.0
Sodium Metasilicate•9H2O: 0.1420
Sodium Phosphate Dibasic•H2O: 70.99
Sodium Selenite: 0.00087
Zinc Sulfate•7H2O: 0.02874

Amino Acids:
L-Alanine 8.90
L-Arginine HCl 63.20
L-Asparagine•H2O: 150
L-Aspartic Acid: 13.31
L-Cysteine•HCl•H2O: 35.13
L-Glutamic Acid: 14.71
L-Glutamine: 146.15
Glycine: 7.51
L-Histidine•HCl•H2O: 20.97
L-Isoleucine: 13.12
L-Leucine: 39.35
L-Lysine•HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 5.76
L-Serine: 31.53
L-Threonine: 35.74
L-Tryptophan: 6.13
L-Tyrosine•Na2•2H2O: 11.35
L-Valine: 35.13

Vitamins:
Biotin: 0.00730
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.00512
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.477
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337


Other:
Adenine HCl: 0.172
D-Glucose: 1441.0
HEPES: 7149.0
Linoleic Acid: 0.0841
Lipoic Acid: 0.00206
Phenol Red, Sodium: 1.242
PutrescinE•2HCl: 0.00016
Sodium Pyruvate: 55.00
Thymidine: 0.0727
Total: 17.6g/liter

Culture Media; Media-Cell Culture; MCDB