RPMI 1640 w/o L-Proline Culture Media

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

< 1 EU/ml

Pale pink, homogenous, free flowing powder

9.0

Magenta, clear, complete

Dissolve 10.373g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Components shown as g/liter

Inorganic Salts:
Calcium Nitrate•4H2O 0.1
Magnesium Sulfate 0.04884
Potassium Chloride 0.4
Sodium Chloride 6.0
Sodium Phosphate Dibasic 0.8

Amino Acids
L-Arginine 0.2
L-Asparagine 0.05
L-Aspartic Acid 0.02
L-Cystine•2HCl 0.0652
L-Glutamic Acid 0.02
L-Glutamine 0.3
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine•HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline Absent
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine•2Na•2H2O 0.02883
L-Valine 0.02

Vitamins
p-Aminobenzoic Acid 0.001
D-Biotin 0.0002
Choline Chloride 0.003
Cyanocobalamin 0.000005
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.00025
Pyridoxine•HCl 0.001
Riboflavin 0.0002
Thiamine•HCl 0.001

Other
D-Glucose 2.0
Glutathione (reduced) 0.001
Phenol Red, sodium 0.0053

Total: 10.373g/liter

Culture Media; Media-Cell Culture; RPMI