Nutrient Mixture Ham's F-12 w/o L-Glutamine, Glucose Culture Media

Off-white to pale orange, homogeneous and free flowing powder

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

≤ 1EU/ml

8.5

Pink, clear, complete

Dissolve 8.68g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g/L sodium bicarbonate. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts
Calcium Chloride: 0.0332
Cupric Sulfate•H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride: 0.05722
Potassium Chloride: 0.2236
Sodium Chloride: 7.599
Sodium Phosphate Dibasic: 0.142
Zinc Sulfate•7H2O: 0.00086
Amino Acids
L-Alanine: 0.0089
L-Arginine•HCl: 0.211
L-Asparagine•H2O: 0.015
L-Aspartic Acid: 0.013
L-Cysteine•HCL•H2O: 0.035
L-Glutamic Acid: 0.0147
L-Glutamine: Absent
Glycine: 0.0075
L-Histidine•HCl•H2O: 0.021
L-Isoleucine: 0.004
L-Leucine: 0.013
L-Lysine•HCl: 0.0365
L-Methionine: 0.0045
L-Phenylalanine: 0.005
L-Proline: 0.0345
L-Serine: 0.0105
L-Threonine: 0.012
L-Tryptophan: 0.002
L-Tyrosine•2Na•2H2O: 0.0078
L-Valine: 0.0117
Vitamins
Biotin: 0.000007
Choline Chloride: 0.014
Vitamin B-12: 0.0014
Folic Acid: 0.0013
i-Inositol: 0.018
Niacinamide: 0.000037
D-Pantothenic Acid, Ca: 0.00004
Pyridoxine•HCl: 0.00006
Riboflavin: 0.00004
Thiamine•HCl: 0.0003
Other
Thymidine: 0.0007
Glucose: Absent
Hypoxanthine: 0.00477
Linoleic Acid: 0.00008
Lipoic (Thioctic) Acid: 0.00021
Phenol Red, Sodium: 0.0013
Putrescine•2HCl: 0.000161
Sodium Pyruvate: 0.11
Total: 8.68g/liter

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's