RPMI 1640 Medium w/L-Glutamine, HEPES w/o Arginine, Lysine Culture Media

Pale orange, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

<1EU/ml

6.7

Orange, clear, complete

Dissolve 16.11g in 900ml of distilled/deionized (DDI) water, stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron membrane. Aliquot into sterile containers.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Inorganic Salts:
Calcium Nitrate•4H2O 100
Magnesium Sulfate 48.84
Potassium Chloride 400
Sodium Chloride 6000
Sodium Phosphate Dibasic 800


Amino Acids:
L-Arginine Absent
L-Asparagine 50
L-Aspartic Acid 20
L-Cystine•2HCl 65.2
L-Glutamic Acid 20
L-Glutamine 300
Glycine 10
L-Histidine 15
Hydroxy-L-proline 20
L-Isoleucine 50
L-Leucine 50
L-Lysine•HCl Absent
L-Methionine 15
L-Phenylalanine 15
L-Proline 20
L-Serine 30
L-Threonine 20
L-Tryptophan 5
L-Tyrosine•Na2•2H2O 28.83
L-Valine 20

Vitamins
P-Aminobenzoic Acid 1
D-Biotin 0.2
Choline Chloride 3
Vitamin B-12 0.005
Folic Acid 1
i-Inositol 35
Niacinamide 1
D-Pantothenic Acid•Ca 0.25
Pyridoxine•HCl 1
Riboflavin 0.2
Thiamine•HCl 1

Other:

D-Glucose 2000
Glutathione 1
HEPES 5960
Phenol Red, Sodium 5

16.11g/L

Culture Media; Media-Cell Culture; RPMI