Dulbecco's MEM Culture Media

Light tan to orange, homogeneous, free-flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

1EU/ml

7.05

Red, clear, complete

Orange, homogeneous, free-flowing powder.

Dissolve 8.53g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride•2H2O: 0.265 g/Liter
Ferric Nitrate•9H2O: 0.0001 g/Liter
Magnesium Sulfate: 0.09767 g/Liter
Potassium Chloride: Absent
Sodium Chloride: 6.4 g/Liter
Sodium Phosphate Monobasic: 0.109 g/Liter

Amino Acids:
L-Arginine•HCl: 0.084 g/Liter
L-Cystine•2HCl: 0.0626 g/Liter
L-Glutamine: 0.584 g/Liter
Glycine: 0.03 g/Liter
L-Histidine•HCl•H2O: 0.042 g/Liter
L-Isoleucine: 0.105 g/Liter
L-Leucine: Absent g/Liter
L-Lysine•HCl: 0.146 g/Liter
L-Methionine: 0.03 g/Liter
L-Phenylalanine: 0.066 g/Liter
L-Serine: 0.042 g/Liter
L-Threonine: 0.095 g/Liter
L-Tryptophan: 0.016 g/Liter
L-Tyrosine•2Na•2H2O: 0.10379 g/Liter
L-Valine: 0.094 g/Liter

Vitamins
Choline Chloride: 0.004
Folic Acid: 0.004
myo-Inositol: 0.0072
Niacinamide: 0.004
D-Pantothenic Acid•Ca: 0.004
Pyridoxal•HCl: 0.004
Riboflavin: 0.0004
Thiamine•HCl: 0.004

Other:
D-Glucose: Absent
Phenol Red, Sodium: 0.0159
Pyruvic Acid, Sodium: 0.11

Total: 8.53g/Liter

Culture Media; Media-Cell Culture; DMEM