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Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652748

Drop out Mix Synthetic-Minus Leucine, Tryptophan, Adenine is used as an addition to Drop-out Base for developing synthetic, drop-out media formulations for selecting auxotrophic cells. More details

$320 / 100 g +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base, Culture Media
Full Product Name
Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base (Powder)
Form/Format
White, homogeneous, free flowing powder
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time
pH
4.2
Solubility
Colorless, clear, complete
Directions per Liter (Broth)
Mix 1.47 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 1.47 grams of drop-out mix with 43.7g MBS653105 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15psi).
Media Formulation
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus leucine, tryptophan, uracil

Amino Acids
Alanine 2.0g
p-Aminobenzoic acid 0.2g
Arginine 2.0g
Asparagine 2.0g
Aspartic acid 2.0g
Cysteine 2.0g
Glutamic acid 2.0g
Glutamine 2.0g
Glycine 2.0g
Histidine 2.0g
Isoleucine 2.0g
Lysine 2.0g
Methionine 2.0g
Phenylalanine 2.0g
Proline 2.0g
Serine 2.0g
Threonine 2.0g
Tyrosine 2.0g
Valine 2.0g

Nucleotide Bases
Adenine 0.5g
Inositol 2.0g

Use: 1.47g/liter 100g makes ~50 liters.

Product Categories/Family for Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base purchase

MBS652748 is a culture media targeting Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base. Also known as Drop-out Mix Synthetic Minus Leucine, Tryptophan, Uracil w/o Yeast Nitrogen Base (Powder).
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