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Edinburgh Minimal Media Phosphate Free Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652754

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L… More details

$235 / 100 g +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Edinburgh Minimal Media Phosphate Free, Culture Media
Full Product Name
Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Store powdered media ar RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
pH
6.5
Solubility
Colorless, clear, complete
Appearance
White, homogenous, free flowing powder.
Directions per Liter
Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
Base
Ammonium Chloride: 5.000
Glucose: 20.000
Sodium Acetate•3H2O: 2.200
Salt Stock
Calcium Chloride•2H2O: 0.0147
Magnesium Chloride•6H2O: 1.050
Potassium Chloride: 1.000
Sodium Sulfate: 0.040
Vitamin Stock
Biotin: 0.00001
Inositol: 0.010
Nicotinic Acid: 0.010
Pantothenic Acid•Ca: 0.001
Mineral Stock
Boric Acid: 0.0005
Citric Acid: 0.001
Copper Sulfate•5H2O: 0.00004
Ferrous Chloride: 0.0002
Manganese Sulfate: 0.0004
Potassium Iodide: 0.0001
Molybdic Acid: 0.00004
Zinc Sulfate•7H2O: 0.0004
Total
29.328g/Lit
Note
The above formulation was derived from the following referenced formula
Edinburgh Minimal Medium (EMM 2)
3g/L Potassium hydrogen phthalate (14.7mM)
2.2g/L Sodium phosphate dibasic (15.5mM)
5g/L Ammonium chloride (93.5mM)
2% (w/v) Glucose (111mM)
20ml/L Salts (50X stock)
1ml/L Vitamins (1000X stock)
0.1ml/L Minerals (10,000X stock)
50X Salts
52.5g/L Magnesium chloride•6H2O (0.26M)
0.735g/L Calcium chloride•2H2O (4.99mM)
50g/L Potassium chloride (0.67M)
2g/L Sodium sulfate (14.1mM)
1000X Vitamins
1g/L Pantothenic acid (4.20mM)
10g/L Nicotinic acid (81.2mM)
10g/L Inositol (55.5mM)
10mg/L Biotin (40.8uM)
10,000X Minerals
5g/L Boric acid (SO.9mM)
4g/L Manganese sulfate (23.7mM) )
4g/L Zinc sulfate•7H20 (13.9mM) )
2g/L Ferrous Chloride (7AOmM) )
0.4g/L Molybdic acid (2A7mM) )
1 g/L Potassium iodide (6.02mM) )
0.4g/L Cupric sulfate.5H20 (1.60mM) )
10g/L Citric acid (47.6mM)

Product Categories/Family for Edinburgh Minimal Media Phosphate Free culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Edinburgh Minimal Media Phosphate Free culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Edinburgh Minimal Media Phosphate Free purchase

MBS652754 is a culture media targeting Edinburgh Minimal Media Phosphate Free. Also known as Edinburgh Minimal Media Phosphate Free (EMMP) (Powder).
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