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Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652759

Drop-out Mix Synthetic, w/o Cystine, Methionine, Uracil w/o Yeast Nitrogen Base (Powder) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics More details

$320 / 100 g +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base, Culture Media
Full Product Name
Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base (Powder)
Form/Format
White, homogeneous, free flowing powder; Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases minus Cystine, Methionine, Uracil:
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
pH
5.0
Solubility
Colorless, clear, complete
Directions per Liter (Broth)
Mix 1.77 grams of drop-out mix with 6.7g Y2025 Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 1.77 grams of drop-out mix with 43.7g MBS653105 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15psi)
Formulation
Amino Acids: Alanine - 2.0g
p-Aminobenzoic - acid 0.2g
Arginine - 2.0g
Asparagine - 2.0g
Aspartic acid - 2.0g
Cysteine - Absent
Glutamic acid - 2.0g
Glutamine - 2.0g
Glycine - 2.0g
Histidine - 2.0g
Isoleucine - 2.0g
Leucine - 10.0g
Lysine - 2.0g
Methionine - Absent
Phenylalanine - 2.0g
Proline - 2.0g
Serine - 2.0g
Threonine - 2.0g
Tryptophan - 2.0g
Tyrosine - 2.0g
Valine - 2.0g

Nucleotide Bases:
Adenine - 2.0g
Inositol - 2.0g
Uracil - Absent

Use 1.77g/liter
100g makes ~50 liters
Important Note
Hygroscopic. Keep tightly closed and desiccated.

Product Categories/Family for Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base purchase

MBS652759 is a culture media targeting Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base. Also known as Drop-out Mix Synthetic, minus Cysteine, Methionine, Uracil w/o Yeast Nitrogen Base (Powder).
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