Letheen Broth Base, AOAC Culture Media

Letheen Broth Base is used with Polysorbate 80 for the testing of quaternary ammonium compounds for antimicrobial acitivity. Polysorbate 80 is not supplied.

Letheen Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing. Quisno, Gibby and Foter discovered that adding lecithin and Polysorbate 80 to FDA Broth results in a medium that neutralized high concentrations of quaternary ammonium salts (2). he resulting medium, termed Letheen (a combination of Lecithin and Tween) was easy to prepare and clear in appearance, aiding to visual inspection for growth. Letheen Broth Base is recommended by the Association of Official Analytical Chemists (AOAC) for use with disinfectants containing cationic surface materials.
Letheen Broth Base is specified for use by the American Society for Testing Materials (ASTM) in Standard Test Method for Presernatives in Water-Containg Cosmetivcs (4). Total neutralization of disinfectants is critical. Disinfectant residues can results in false negative (no growth) tests.

Small volumes of Letheen Broth Base, AOAC culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS652766 is a culture media targeting Letheen Broth Base, AOAC. Also known as Letheen Broth Base, AOAC (Powder).