Keratinocyte Basal Medium MCDB 153 w/o Methionine, Cysteine, Calcium Culture Media

MCDB media were designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium was formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promoted growth of a specific cell type. MCDB 105 and 110 are modifications of MCDB 104 medium, optimized for long-term survival and rapid clonal growth of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human foreskin fibroblasts using FBSP or hormone and growth factor supplements. MCDB 151, 153, 201 and 302 are modifications of Ham's nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts and chinese hamster ovary (CHO) cells using low levels of FBSP, extensive trace elements or no serum protein. The selection of a nutrient medium is strongly influenced by 1. type of cell; 2. type of culture (monolayer, suspension, clonal); and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line. MCDB 153 is a basal medium for the growth of normal keratinocytes and squamous epithelial cells after growth factor supplementation. Calcium content is low. Supplement with growth factors or fetal calf serum. MBS652767 is a basal medium lacking methionine and cysteine.

Small volumes of Keratinocyte Basal Medium MCDB 153 w/o Methionine, Cysteine, Calcium culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS652767 is a culture media targeting Keratinocyte Basal Medium MCDB 153 w/o Methionine, Cysteine, Calcium. Also known as Keratinocyte Basal Medium MCDB 153 w/o Methionine, Cysteine, Calcium (Powder).