PET Cell Dissociation Reagent Culture Media

Cell Culture Grade

May be stored at 4 degree C for short-term (4-6 weeks). For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Aliquots are stable for 6 months.
Shipping: Ships with Dry Ice.

7.45

12.65mS

539 BAEEunits/ml

No Detection of microbial growth.

After rinsing the cell monolayers using HBS, add 3ml of ice cold PET dissociation reagent. Do not keep the flask in the incubator. Incubate at room temperature and monitor the monolayer by microscopy (preferably phase contrast). When the cells begin to separate, gently remove most of the PET (without removing the cells) and allow the flask to remain at room temperature. To determine whether the cells are ready to harvest, tap the flask (held in an upright position) firmly by hand.
When the cells float free, resuspend them in cold HBS supplemented with 5% Fetal Bovine Serum to stop the tryptic action. Mix well by pipetting the cell suspension, centrifuge and resuspend the cell pellet in the appropriate complete serum-free medium.

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Culture Media; Media-Cell Culture