MCDB 110 Diploid Fibroblast-like Cell Medium Culture Media

White to light orange, homogeneous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Opened bottles should becapped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

White, homogeneous, free flowing powder

1EU/ml

Pale yellow, clear, complete

6.5

Dissolve 15.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Add sodium bicarbonate if required.Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Ammonium Metavandate: 0.000585
Ammonium Molybdate: 0.00124
Calcium Chloride•2H20: 147.00
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.39
Magnesium Sulfate: 120.38
Manganese Sulfate: 0.000151
Nickel Chloride Hexahydrate: 0.00012
Potassium Chloride: 372.75
Sodium Chloride: 6546.00
Sodium Metasilicate•9H2O: 0.1421
Sodium Phosphate Dibasic: 425.94
Sodium Selenite: 0.0038
Stannous Chloride Dihydrate: 0.000113
Zinc Sulfate•7H2O : 0.144

Amino Acids:
L-Alanine: 8.90
L-Arginine HCl: 210.70
L-Asparagine H2O: 15.00
L-Aspartic Acid: 13.31
L-Cysteine HCl: H2O 8.78
L-Glutamic Acid: 14.71
L-Glutamine: 365.30
Glycine: 22.52
L-Histidine HCl: H2O 20.97
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.04
L-Tyrosine: 7.84
L-Valine: 11.72


Vitamins:
Biotin: 0.00733
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.000602
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.2383
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337

Other:
Adenine HCl: 1.72
Thymidine: 0.0727
D-Glucose: 720.64
HEPES Free Acid: 5958.00
Thioctic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine•2HCl: 0.0001611
Sodium Pyruvate: 110.00

Total: 15.3g/liter

Culture Media; Media-Cell Culture; MCDB