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Dulbecco's MEM Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652803

Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The… More details

$305 / 10 L +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dulbecco's MEM, Culture Media
Full Product Name
Dulbecco's MEM (DMEM) Ham's F-12 w/o Calcium, Phenol Red, w/ Ferric Nitrate (Powder)
Form/Format
Off-white, homogeneous, free flowing powder
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Endotoxin Level
< = 1EU/mL
Directions per Liter
Dissolve 11.84g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
pH
7.6
Solubility
Colorless, clear, complete
Components shown as mg/liter
Inorganic Salts
Calcium Chloride Dihydrate Absent
Cupric Sulfate•5H2O 0.0013
Ferric Nitrate•9H2O 0.05
Ferrous Sulfate•7H2O 0.417
Potassium Chloride 311.8
Magnesium Chloride 28.66
Magnesium Sulfate 48.84
Sodium Chloride 6996
Sodium Phosphate Monobasic 54.3
Sodium Phosphate Dibasic 71.0
Zinc Sulfate•7H2O 0.432
Amino Acids

L-Alanine 4.45
L-Arginine 121.97
L-Asparagine•H2O 7.5
L-Aspartic Acid 6.65
L-Cysteine•HCI•H2O 17.56
L-Cystine•2HCI 31.29
L-Glutamic Acid 7.35
L-Glutamine 365
Glycine 18.75
L-Histidine HCl•H2O 31.48
L-Isoleucine 54.47
L-Leucine 59.05
L-Lysine•HCI 91.25
L-Methionine 17.24
L-Phenylalanine 35.48
L-Proline 17.25
L-Serine 26.25
L-Threonine 53.45
L-Tryptophan 9.02
L-Tyrosine 55.79
L-Valine 52.85
Vitamins

Biotin 0.0035
D-Pantothenic Acid•Na 2.24
Choline Chloride 8.98
Cyanocobalamin 0.680
Folic Acid 2.66
i-inositol 12.60
Nicotinamide 2.02
Pyridoxal•HCI 2.00
Pyridoxine•HCI 0.031
Riboflavin 0.219
Thiamine•HCI 2.17
Other

Thymidine 0.365
D-Glucose 3150
Hypoxanthine 2.1
Linoleic Acid 0.042
Lipoic Acid 0.105
Putrescine•HCl 0.081
Sodium Pyruvate 55.05
Phenol Red Absent
Total : 11.84g/L

Other Notes

Small volumes of Dulbecco's MEM culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dulbecco's MEM purchase

MBS652803 is a culture media targeting Dulbecco's MEM. Also known as Dulbecco's MEM (DMEM) Ham's F-12 w/o Calcium, Phenol Red, w/ Ferric Nitrate (Powder).
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