Dulbecco's MEM Culture Media

Orange, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time

1EU/ml

6.4

Orange, clear, complete

Dissolve 8.85g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Inorganic Salts:
Calcium Chloride, anhy 0.200
Ferric Nitrate 9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic H2O 0.125

Amino Acids:
L-Arginine HCl 0.084
L-Cystine Absent
L-Glutamine 0.584
Glycine 0.03
L-Histidine HCl H2O Absent
L-Isoleucine 0.105
L-Leucine 0.105
L-Lysine HCl 0.146
L-Methionine Absent
L-Phenylalanine 0.066
L-Serine 0.042
L-Threonine 0.095
L-Tryptophan 0.016
L-Tyrosine 2Na 2H2O 0.104
L-Valine 0.094

Vitamins:
Choline Chloride 0.004
Folic Acid 0.004
i-Inositol 0.0072
Niacinamide 0.004
D-Pantothenic Acid Ca 0.004
Pyridoxal HCl 0.004
Riboflavin 0.0004
Thiamine HCl 0.004

Other:
D-Glucose Absent
Phenol Red, Sodium 0.015
Pyruvic Acid, Sodium 0.110

Total: 8.85g/Liter

Culture Media; Media-Cell Culture; DMEM