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MEM Eagle w/Earle's, w/o L-Glutamine, amino acids Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652807

Minimum Essential Medium (MEM was, developed by Harry Eagle. It is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that these types of cells… More details

$295 / 10 L +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MEM Eagle w/Earle's, w/o L-Glutamine, amino acids, Culture Media
Full Product Name
MEM Eagle w/Earle's, w/o L-Glutamine, amino acids (Powder)
Form/Format
Light orange, homogenous, free flowing powder
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Endotoxin Level
<1EU/ml
pH
5.3
Solubility
Yellow, clear, complete
Directions per Liter
Dissolve 8.70 grams in 900 mL of distilled/deionized (DDI) water, stirring gently until completely solubilized. Do NOT heat. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Filter-sterilize using a 0.22 micron membrane. Add additional water to bring the solution to final volume. Aliquot into sterile containers.
Media Formulation
Components shown as g/liter:
Inorganic Salts:
Calcium Chloride•2H2O 0.265
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Mono Anhy 0.122

Amino Acids:
L-Arginine&bullHCl Absent
L-Cystine&bull2HCl Absent
L-Glutamine Absent
L-Histidine&bullHCl&bullH2O Absent
L-Isoleucine Absent
L-Leucine Absent
L-Lysine&bullHCl Absent
L-Methionine Absent
L-Phenylalanine Absent
L-Threonine Absent
L-Tryptophan Absent
L-Tyrosine&bull2Na&bull2H2O Absent
L-Valine Absent

Vitamins:
Choline Chloride 0.001
Folic Acid 0.001
myo-Inositol 0.002
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.001
Pyridoxal•HCl 0.001
Riboflavin 0.0001
Thiamine•HCl 0.001

Other:
D-Glucose 1
Phenol Red, Sodium 0.011

Total: 8.704g/liter

Other Notes

Small volumes of MEM Eagle w/Earle's, w/o L-Glutamine, amino acids culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEM Eagle w/Earle's, w/o L-Glutamine, amino acids purchase

MBS652807 is a culture media targeting MEM Eagle w/Earle's, w/o L-Glutamine, amino acids. Also known as MEM Eagle w/Earle's, w/o L-Glutamine, amino acids (Powder).
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