Edinburgh Minimal Media, Nitrogen Free Culture Media

White, homogeneous, free flowing powder

Molecular Biology Grade

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Colorless, clear, complete

6.0

Dissolve 27.33g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

The above formulation was derived from the following referenced formula:
Edinburgh Minimal Medium (EMM 2) --
3g/L: Potassium hydrogen phthalate (14.7mM)
2.2g/L: Sodium phosphate dibasic (15.5mM)
5g/L: Ammonium chloride (93.5mM)
2% (w/v): Glucose(111mM)
20ml/L: Salts (50X stock)
1ml/L: Vitamins (1000X stock)
0.1ml/L: Minerals (10,000X stock)
50X Salts --
52.5g/L: Magnesium chloride•6H2O (0.26M)
0.735g/L: Calcium chloride•2H2O (4.99mM)
50g/L: Potassium chloride (0.67M)
2g/L: Sodium sulfate (14.1mM)
1000X Vitamins --
1g/L: Pantothenic acid (4.20mM)
10g/L: Nicotinic acid (81.2mM)
10g/L: Inositol (55.5mM)
10mg/L Biotin (40.8uM)
10,000X Minerals --
5g/L: Boric acid (80.9mM)
4g/L: Manganese sulfate (23.7mM)
4g/L: Zincsulfate•7H2O (13.9mM)
2g/L: Ferric chloride•6H2O (7.40mM)
0.4g/L: Molybdic acid (2.47mM)
1g/L: Potassium iodide (6.02mM)
0.4g/L: Cupric sulfate.5H2O (1.60mM)
10g/L: Citric acid (47.6mM)

Base --
Potassium Hydrogen Phthalate: 3.00
Sodium Phosphate Dibasic: 2.20
Ammonium Chloride: Absent
Glucose: 20.00
Salt Stock --
Magnesium Chloride•6H2O: 1.05
Calcium Chloride•2H2O: 0.0147
Potassium Chloride: 1.00
Sodium Sulfate: 0.04
Vitamin Stock --
Pantothenic Acid: 0.001
Nicotinic Acid: 0.01
Inositol: 0.01
Biotin: 0.00001
Mineral Stock: --
Boric Acid: 0.0005
Manganese Sulfate: 0.0004
Zinc Sulfate•7H2O: 0.0004
Ferrous Chloride: 0.0002
Molybdic Acid: 0.00004
Potassium Iodide: 0.0001
Copper Sulfate •5H2O: 0.00004
Citric Acid: 0.001
Total: 27.33g/Liter

Culture Media; Media-Yeast